Cell Signaling Technology

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Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4410

secondary  

Applications Species-Reactivity Sensitivity MW (kDa) Isotype
F Goat
IF-IC

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

F(ab')2 fragments are prepared from goat antibodies that have been adsorbed against human IgG and human serum.

F(ab’)2片段是采用羊抗体制备的,已经吸附了人类IgG和人血清。

Description

Anti-Mouse IgG (H+L) F(ab')2 Fragment was conjugated to Alexa Fluor® 647 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab')2 fragment results in less non-specific binding to cells through Fc receptors.

Anti-Mouse IgG (H+L) F(ab’)2 Fragment在最佳条件下结合了 Alexa Fluor ® 647荧光染料,浓度为2mg/ml. F(ab’)2片段可以减少通过Fc受体的非特异性结合。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of untreated Jurkat cells using Akt (5G3) Mouse mAb #2966 detected with Anti-Mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) (green) compared to a nonspecific negative control antibody (red).流式细胞术分析未处理的Jurkat细胞,所用抗体为Akt (5G3) Mouse mAb #2966,检测抗体为Anti-Mouse IgG (H+L), F(ab’)2Fragment (Alexa Fluor ® 647 Conjugate) (绿色) ,与非特异性阴性对照抗体(红色)作对比。

High Content Screening

High Content Screening

High content analysis of C2C12 cells exposed to varying concentrations of U0126 #9903 for 2 hours. With increasing concentrations of U0126, a significant decrease (~20 fold) in phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) signal as compared to the untreated control was observed. When using Phospho-p44/42 MAPK (Erk1/2) protein as a measurement, the IC50 of this compound was 2.5 μM. Data was generated on the Acumen® HCS platform using Anti-Mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate).高通量分析C2C12细胞,该细胞暴露于LY294002 #9901 2个小时。与未处理的对照组相比较,随着LY294002 浓度的增加phospho-S6 Ribosomal protein (Ser202/204)信号显著降低(约20倍)。当采用 Phospho-p44/42 MAPK (Erk1/2)检测时,IC50为2.5mM。数据时以Acumen ® HCS为平台生成的,所用抗体为Anti-Mouse IgG (H+L), F(ab’)2 Fragment (Alexa Fluor ® 647 Conjugate)。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Pan-Keratin (C11) Mouse mAb #4545 detected with Anti-Mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) (blue). Red = propidium iodide (fluorescent DNA dye).激光共聚焦免疫荧光分析HeLa细胞,所用抗体为g Pan-Keratin (C11) Mouse mAb #4545检测抗体为anti-Mouse IgG (H+L), F(ab’)2 Fragment (Alexa Fluor ® 647 Conjugate) (蓝色)。 红色为propidium iodide,荧光DNA染料。

Applications

This product has been optimized for use as a secondary antibody in immunofluorescent applications.

本产品已经过优化,可以作为二抗应用于免疫荧光中。

Directions for Use

The optimal dilution of the anti-species antibody should be determined for each application. However, a final dilution of 1:1000 should yield acceptable results for most immunofluorescent applications.

抗体的最佳稀释度取决于每一个应用。然而,1:1000的终稀释度在免疫荧光分析中可以得到好的结果。

Background

Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.

荧光抗IgG结合物适用于流式细胞术和免疫荧光。CST公司的严格的质控程序保证了每一个结合物具有最佳的特异性和荧光强度。

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Acumen is a registered trademarks of TTP Labtech.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation, for research use only for immunocytometry, immunohistochemistry, high content screening (HCS) analysis, or flow cytometry applications.

The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer (whether the buyer is an academic or for-profit entity), for Immunocytochemistry, high content screening (HCS) analysis, or flow cytometry applications. The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) resale, whether or not such product or its components are resold for use in research; or for any other commercial purpose. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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