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Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate) #4409


Applications Species-Reactivity Sensitivity MW (kDa) Isotype
HCA Goat

Species cross-reactivity is determined by western blot.

Applications Key: HCA=High Content Analysis, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

F(ab')2 fragments are prepared from goat antibodies that have been adsorbed against human IgG and human serum.



Anti-Mouse IgG (H+L) F(ab')2 Fragment was conjugated to Alexa Fluor® 555 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab')2 fragment results in less non-specific binding to cells through Fc receptors.

Anti-Mouse IgG (H+L) F(ab’)2 Fragment在最佳条件下结合了 Alexa Fluor ® 555荧光染料,浓度为2mg/ml. F(ab’)2片段可以减少通过Fc受体的非特异性结合。



Confocal immunofluorescent analysis of HeLa cells using S6 Ribosomal Protein (54D2) Mouse mAb #2317 detected with Anti-Mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate) (red, left) compared to an isotype control (right). Actin filaments have been labeled with fluorescein phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦免疫荧光分析HeLa细胞,所用抗体为S6 Ribosomal Protein (54D2) Mouse mAb #2317,检测抗体为anti-Mouse IgG (H+L), F(ab’)2 Fragment (Alexa Fluor ® 555 Conjugate) (上图),与同型对照(下图)作比较。 肌动蛋白丝采用fluorescein phalloidi标记。蓝色伪彩为荧光DNA染料,信息为DRAQ5 ® #4084。

High Content Screening

High Content Screening

High content analysis of C2C12 cells exposed to varying concentrations of LY294002 #9901 for 2 hours. With increasing concentrations of LY294002, a significant decrease (~20 fold) in phospho-S6 Ribosomal protein (Ser235/236) signal as compared to the untreated control was observed. When using phospho-S6 Ribosomal protein as a measurement, the IC50 of this compound was 2.3 μM. Data was generated on the Acumen® HCS platform using Anti-Mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate).高通量分析C2C12细胞,该细胞暴露于LY294002 #9901 2个小时。与未处理的对照组相比较,随着LY294002 浓度的增加phospho-S6 Ribosomal protein (Ser235/236)信号显著降低(约20倍)。当采用 phospho-S6 Ribosomal protein 检测时,IC50为2.3mM。数据时以Acumen ® HCS为平台生成的,所用抗体为Anti-Mouse IgG (H+L), F(ab’)2 Fragment (Alexa Fluor ® 555 Conjugate)。


This product has been optimized for use as a secondary antibody in immunofluorescent applications.


Directions for Use

The optimal dilution of the anti-species antibody should be determined for each application. However, a final dilution of 1:1000 should yield acceptable results for most immunofluorescent applications.



Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.


Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Acumen is a registered trademarks of TTP Labtech.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation, for research use only for immunocytometry, immunohistochemistry, high content screening (HCS) analysis, or flow cytometry applications.

The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer (whether the buyer is an academic or for-profit entity), for Immunocytochemistry, high content screening (HCS) analysis, or flow cytometry applications. The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) resale, whether or not such product or its components are resold for use in research; or for any other commercial purpose. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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