Cell Signaling Technology

Product Pathways - Autophagy Signaling

BNIP3 (D7U1T) Rabbit mAb #44060

No. Size Price
44060S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
44060 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 22-28, 50-55 Rabbit IgG
IP 1:100
IHC-P 1:100
IF-IC 1:800

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

BNIP3 (D7U1T) Rabbit mAb recognizes endogenous levels of total BNIP3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human BNIP3 protein.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with cobalt chloride (100 μM, 24 hr; right), using BNIP3 (D7U1T) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalliodin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa or MCF7 cells, untreated (-) or cobalt chloride-treated (100 μM, overnight; +), using BNIP3 (D7U1T) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

IP

IP

Immunoprecipitation of BNIP3 from MCF7 cells treated with cobalt chloride (100 μM; overnight). Lane 1 represents 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is BNIP3 (D7U1T) Rabbit mAb. Western blot analysis was performed using BNIP3 (D7U1T) Rabbit mAb. A conformation-specific secondary antibody was used to avoid reactivity with IgG.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded normal human liver using BNIP3 (D7U1T) Rabbit mAb in the presence of control peptide (left) and antigen-specific peptide (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded MCF7 cell pellets, untreated (left) or cobalt chloride-treated (right), using BNIP3 (D7U1T) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full-length human BNIP3 protein (hBNIP3-Myc; +), using BNIP3 (D7U1T) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using BNIP3 (D7U1T) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using BNIP3 (D7U1T) Rabbit mAb.

Background

BNIP3 (Bcl-2/E1B-19kDa interacting protein 3) is a pro-apoptotic mitochondrial protein and Bcl-2 family member that contains a Bcl-2 homology 3 (BH3) domain and a carboxyl-terminal transmembrane (TM) domain (1-3). While BNIP3 has a predicted molecular weight of about 22 kDa, it runs anomalously on SDS-PAGE and includes a band of around 60 kDa that may be a dimeric form that is not reduced (2). BNIP3 associates with anti-apoptotic family members Bcl-2, Bcl-xL, and the adenovirus homologue E1B-19kDa. BNIP3 is distinct from other Bcl-2 family members that contain only the BH3 domain in that the TM domain, and not the BH3 domain, is required for mitochondrial targeting and pro-apoptotic activity (4). In addition to apoptosis, BNIP3 has been implicated in necrosis (5) and autophagy (6-11). In hypoxic conditions, BNIP3 can induce mitochondrial autophagy (mitophagy) by disrupting the Bcl-2-Beclin-1 complex (9). BNIP3 can also promote mitophagy by triggering the translocation of the E3 ubiquitin ligase Parkin to the mitochondria (10) or by directly binding LC3 on the autophagosome (11). BNIP3 may also localize to the endoplasmic reticulum (ER) where it can selectively induce the autophagic clearance of ER (ERphagy) (11). Increased expression of BNIP3 under hypoxic conditions is mainly regulated by the transcription factor HIF-1α (12-14). Silencing of the BNIP3 promoter by methylation has been observed in several types of cancer cells and may play an important role in their survival (14-18).

  1. Boyd, J.M. et al. (1994) Cell 79, 341-51.
  2. Chen, G. et al. (1997) J Exp Med 186, 1975-83.
  3. Yasuda, M. et al. (1998) J Biol Chem 273, 12415-21.
  4. Ray, R. et al. (2000) J Biol Chem 275, 1439-48.
  5. Vande Velde, C. et al. (2000) Mol Cell Biol 20, 5454-68.
  6. Daido, S. et al. (2004) Cancer Res 64, 4286-93.
  7. Tracy, K. et al. (2007) Mol Cell Biol 27, 6229-42.
  8. Quinsay, M.N. et al. (2010) Autophagy 6, 855-62.
  9. Bellot, G. et al. (2009) Mol Cell Biol 29, 2570-81.
  10. Lee, Y. et al. (2011) Am J Physiol Heart Circ Physiol 301, H1924-31.
  11. Hanna, R.A. et al. (2012) J Biol Chem 287, 19094-104.
  12. Bruick, R.K. (2000) Proc Natl Acad Sci USA 97, 9082-7.
  13. Guo, K. et al. (2001) Cell Death Differ 8, 367-76.
  14. Sowter, H.M. et al. (2001) Cancer Res 61, 6669-73.
  15. de Angelis, P.M. et al. (2004) Int J Oncol 24, 1279-88.
  16. Okami, J. et al. (2004) Cancer Res 64, 5338-46.
  17. Murai, M. et al. (2005) Clin Cancer Res 11, 1021-7.
  18. Murai, M. et al. (2005) Br J Cancer 92, 1165-72.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

SignalStain is a trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications