Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Phospho-Jak2 (Tyr1007) (D15E2) Rabbit mAb #4406

No. Size Price
4406S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4406T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
4406 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 125 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Rat, Monkey, Bovine,

Specificity / Sensitivity

Phospho-Jak2 (Tyr1007) (D15E2) Rabbit mAb detects endogenous levels of Jak2 when phosphorylated at Tyr1007. This antibody can also detect double phosphorylation at Tyr1007 and Tyr1008. Cross-reactivity may be observed with phosphorylated Jak3 and Tyk2.

Phospho-Jak2 (Tyr1007) (D15E2) Rabbit mAb只能检测内源的在Tyr1007位点磷酸化的Jak2蛋白。此抗体也可检测在Tyr1007 和 Tyr1008位点双磷酸化的Jak2。抗体也可与Jak3 和 Tyk2 蛋白交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1007 of human Jak2.

此单克隆抗体是通过合成人源对应的Jak2 Tyr1007位点周围的肽段来免疫动物而获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from UT-7 cells, untreated or treated with erythropoietin (EPO; 3 units/ml; 5 min) and from untreated HEL cells, using Phospho-Jak2 (Tyr1007) (D15E2) Rabbit mAb (upper) or total Jak2 (D2E12) Rabbit mAb #3230 (lower). HEL cells contain a V617F activating mutation in Jak2.Western免疫印迹。用Phospho-Jak2 (Tyr1007) (D15E2) Rabbit mAb (上图) 或 total Jak2 (D2E12) Rabbit mAb #3230 (下图) 研究经erythropoietin (EPO; 3 units/ml; 5 min)处理的UT-7细胞和未经处理的UT-7细胞及HEL细胞的细胞提取液。


Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3 and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4) and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.

Janus家族中的酪氨酸激酶成员(Jak1, Jak2, Jak3和Tyk2)通过配体结合到一些相关的细胞因子受体上而被激活(1)。除了细胞因子受体的激活方式,Jak蛋白能自身磷酸化和磷酸化与其相关的受体从而为信号蛋白提供多个结合位点。这些信号蛋白如Stats (2), Shc (3), 胰岛素受体底物(4) and 成簇黏附激酶 (FAK) (5)都是典型的含有SH2 或者其它的磷酸化酪氨酸结合区域。

  1. Leonard, W.J. and O'Shea, J.J. (1998) Annu Rev Immunol 16, 293-322.
  2. Darnell, J.E. (1997) Science 277, 1630-5.
  3. VanderKuur, J. et al. (1995) J Biol Chem 270, 7587-93.
  4. Argetsinger, L.S. et al. (1995) J Biol Chem 270, 14685-92.
  5. Zhu, T. et al. (1998) J Biol Chem 273, 10682-9.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Jak antibodies produced under license (granting certain rights including those under U.S. Patent No. 5,658,791) from Chemicon International, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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