Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-EGF Receptor (Tyr1148) Antibody #4404

EGF   EGFR   epidermal growth factor receptor   Her1  

No. Size Price
4404S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4404 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 175 Rabbit
IHC-P 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin),

Homology

Species predicted to react based on 100% sequence homology: Rat,

Specificity / Sensitivity

Phospho-EGF Receptor (Tyr1148) Antibody detects endogenous levels of EGF receptor only when phosphorylated at tyrosine 1148. This antibody does not cross-react with other tyrosine-phosphorylated ErbB family members. 磷酸化EGFR (Tyr1148)的抗体仅在EGFR的Tyr1148位点被磷酸化后才能检测到内源EGFR的水平。本抗体不与其它酪氨酸位点被磷酸化的ErbB家族蛋白发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1148 of human EGF receptor. Antibodies are purified by protein A and peptide affinity chromatography. 多克隆抗体通过免疫动物得到,该磷酸化多肽是根据人的EGFR蛋白Tyr1148附近的氨基酸序列合成的。抗体经过protein A和亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of various cell lysates containing activated EGFR, ErbB2, ErbB3 and ErbB4 proteins, using Phospho-EGF Receptor (Tyr1148) Antibody (upper) or Phospho-Tyrosine mAb (P-Tyr-100) #9411 (lower). 用抗磷酸化EGFR (Tyr1148)的抗体(上)或抗磷酸化酪氨酸(P-Tyr-100)的抗体#9411(下)对含有活化的EGFR, ErbB2, ErbB3 和 ErbB4蛋白的各种细胞裂解物进行免疫印迹检测。

Western Blotting

Western Blotting

Western blot analysis of extracts from A431 cells, untreated or EGF-stimulated, using Phospho-EGF Receptor (Tyr1148) Antibody (upper) or EGF Receptor Antibody #2232 (lower). 用抗磷酸化EGFR (Tyr1148)的抗体(上)或抗EGFR的抗体#2232(下)对经过或未经EGF处理的A431细胞进行免疫印迹检测。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical staining of phosphorylated EGF Receptor in paraffin-embedded human breast carcinoma, showing membrane localization using Phospho-EGF Receptor (Tyr1148) Antibody. 用抗磷酸化EGFR (Tyr1148)的抗体对石蜡包埋的人乳腺癌组织中磷酸化的EGFR进行免疫组化检测,图中显示胞膜阳性。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded MDA-MB-468 cells, untreated (left), EGF-treated (middle) or EGF and Tarceva®- treated (right), using Phospho-EGF Receptor (Tyr1148) Antibody. 用抗磷酸化EGFR (Tyr1148)的抗体对经过(中)、未经(左)EGF处理或经过EGF和Tarceva®同时处理(右)的石蜡包埋的MDA-MB-468细胞进行免疫组化检测。

Background

The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation (1,2). Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins (3,4). c-Src is involved in phosphorylation of EGFR at Tyr845 (5). The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling (6). Phosphorylation of EGFR at Tyr1045 creates a major docking site for c-Cbl, an adaptor protein that leads to receptor ubiquitination and degradation following EGFR activation (7,8). The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 (9). A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provides a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation (2). Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation (10).

表皮生长因子(EGF)受体是一个属于HER/ErbB蛋白家族的跨膜酪氨酸激酶。配体的结合导致了受体的二聚化,自磷酸化,下游信号分子的激活,内化以及溶酶体降解 (1,2)。表皮生长因子受体(EGFR)在激酶域Tyr845位点的磷酸化与稳定激活回路有关,并且维持了酶的激活状态,为底物蛋白提供了可结合的表位(3,4)。c-Src与 EGFR 在 Tyr845位点的磷酸化有关(5)。PLCγ的SH2结构域结合到磷酸化的Tyr992位点,将引起PLCγ介导的下游信号通路的激活(6)。EGFR Tyr1045位点的磷酸化则为c-Cbl生成一个主要的停靠位点,这个衔接蛋白将导致受体的泛素化以及EGFR活化后的降解(7,8)。GRB2衔接蛋白则结合到活化后的EGFR磷酸化的Tyr1068位点(9)。一对磷酸化EGFR残基(Tyr1148 and Tyr1173)为Shc支架蛋白提供了停靠点,这两个位点都与MAPK激酶通路的激活有关(2)。EGFR在特定的丝氨酸和苏氨酸残基上的磷酸化减弱了EGFR激酶的活性。EGFR羧基末端残基Ser1046 和 Ser1047被CaM 激酶II磷酸化,这两个丝氨酸任何一个突变都将导致EGFR酪氨酸自身磷酸化的上调(10)。

  1. Hackel, P.O. et al. (1999) Curr Opin Cell Biol 11, 184-9.
  2. Zwick, E. et al. (1999) Trends Pharmacol Sci 20, 408-12.
  3. Cooper, J.A. and Howell, B. (1993) Cell 73, 1051-4.
  4. Hubbard, S.R. et al. (1994) Nature 372, 746-54.
  5. Biscardi, J.S. et al. (1999) J Biol Chem 274, 8335-43.
  6. Emlet, D.R. et al. (1997) J Biol Chem 272, 4079-86.
  7. Levkowitz, G. et al. (1999) Mol Cell 4, 1029-40.
  8. Ettenberg, S.A. et al. (1999) Oncogene 18, 1855-66.
  9. Rojas, M. et al. (1996) J Biol Chem 271, 27456-61.
  10. Feinmesser, R.L. et al. (1999) J Biol Chem 274, 16168-73.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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