Cell Signaling Technology

Product Pathways - Motif Antibodies

Phospho-(Ser/Thr) PKD Substrate Antibody #4381

general   motif   PBS   PKCmu   PKD   substrate  

No. Size Price
4381S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4381 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 All Species Expected, Endogenous Rabbit
E-P 1:1000

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, E-P=Peptide ELISA (DELFIA),

Specificity / Sensitivity

Phospho-(Ser/Thr) PKD Substrates Antibody detects peptides and proteins containing a phospho-Ser/Thr residue with arginine at the -3 position and leucine at the -5 position, prefering proline at the -1 position within the motif. The antibody may cross-react with some peptides and proteins containing an arginine residue at -3 position and a hydrophobic or basic residue at the -5 position. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Phospho-(Ser/Thr) PKD Substrates Antibody识别包含-3位上有精氨酸和-5位上有亮氨酸的磷酸化丝氨酸/苏氨酸残基的肽段或蛋白,最好在-1位上还有脯氨酸。此抗体可能与包含-3位精氨酸和-5位疏水或碱性氨基酸的肽段或蛋白有交叉反应。(美国专利号:6,441,140、6,982,318、7,259,022、7,344,714;U.S.S.N. 11,484,485;及所有国外相应专利)

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phospho-PKD substrate peptide. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or PMA-treated (100 nM), using PKD Substrates Antibody. Arrows point to PMA-inducible bands. (Provided by Dr. Alex Toker, Beth Israel Deaconess Medical Center, Harvard Medical School).

对PC12细胞,未处理或100nM PMA处理,使用PKD Substrates Antibody进行Western blot分析。箭头指示PMA诱导的条带。(Dr. Alex Toker, Beth Israel Deaconess Medical Center, Harvard Medical School提供)



Phospho-(Ser/Thr) PKD Substrates Antibody DELFIA® Assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (T* and S* denote phosphorylated threonine and serine.) (DELFIA® is a registered trademark of PerkinElmer, Inc.)

Phospho-(Ser/Thr) PKD Substrates Antibody DELFIA®分析:磷酸化和非磷酸化的信噪比。(T*和S*代表磷酸化的苏氨酸和丝氨酸)(DELFIA®是PerkinElmer公司的注册商标)

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells transfected with pcDNA or with activated PKD (S744E/S748E), using PKD Substrates Antibody (Arrow points to active PKD as well as inducible bands).

对HeLa细胞,转染pcDNA或激活的pKD(S744E/S748E),使用PKD Substrates Antibody进行Western blot分析(箭头指示激活的PKD)。

Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7 cells, untreated or treated with TPA (#4174, 200 nM, 20 min), using the Phospho-(Ser/Thr) PKD Substrates Antibody.

对COS-7细胞抽提物,未处理或用TPA(#4174, 200 nM, 20 min)处理,使用Phospho-(Ser/Thr) PKD Substrates Antibody进行免疫印迹分析


Protein kinase D1 (PKD1), also called PKCμ, is one member of the protein kinase D family. PKD members are serine/threonine protein kinases implicated in very diverse cellular functions including cell growth and survival, Golgi organization and immune response (1). The information about the natural substrates of PKD is limited (2,3). The enzyme recognizes and phosphorylates the following consensus sequence: LXRXXS/T. Phospho-(Ser/Thr) PKD Substrate Antibody recognizes phosphorylated PKD substrates at their consensus motif, providing a powerful new tool for PKD target discovery and characterization as well as HTS drug screening for potential kinase regulators.

Protein kinase D1 (PKD1),也称为PKCμ,是蛋白激酶D家族的一员。PKD成员是丝氨酸/苏氨酸蛋白激酶,涉及多种细胞内功能包括细胞生长和存活、高尔基体组装和免疫反应等(1)。目前对PKD自然底物的信息了解有限(2,3)。该酶识别并磷酸化一致序列:LXRXXS/T。Phospho-(Ser/Thr) PKD Substrate Antibody识别含共同基序磷酸化的PKD,为PKD目标物的发现和鉴定提供了强大的工具,也为潜在激酶调节者的高通量药物筛选提供了强大的工具。

  1. Rykx, A. et al. (2003) FEBS Lett 546, 81-6.
  2. Nishikawa, K. et al. (1997) J Biol Chem 272, 952-60.
  3. Iglesias, T. et al. (2000) J Biol Chem 275, 40048-56.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

DELFIA is a registered trademark of PerkinElmer, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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