Cell Signaling Technology

Product Pathways - Neuroscience

NeuroD (D35G2) Rabbit mAb #4373

No. Size Price
4373S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
4373 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 49 Rabbit IgG
IP 1:50
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, ChIP=Chromatin IP,

Specificity / Sensitivity

NeuroD (D35G2) Rabbit mAb detects endogenous levels of total NeuroD protein.

NeuroD (D35G2) Rabbit mAb 兔单抗识别内源性的总NeuroD蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Gln15 of human NeuroD protein.

该单克隆抗体由合成的人类NeuroD蛋白Gln15位点附近肽段免疫动物而制成。

Western Blotting

Western Blotting

Western blot analysis of extracts from IMR-32 cells using NeuroD (D35G2) Rabbit mAb.

Western blot分析IMR-32细胞提取物,使用的抗体是NeuroD (D35G2) Rabbit mAb 兔单抗。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 SH-SY5Y cells treated with Human Brain-Derived Neurotrophic Factor (BDNF) #3897 (50 ng/ml) for 30 minutes and either 10 μl of NeuroD (D35G2) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human EGR1 Promoter Primers #5549, human EGR2 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

使用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003试剂盒,将Human Brain-Derived Neurotrophic Factor (BDNF) #3897 (50ng/ml)处理30分钟的4 x 10^6 SH-SY5Y细胞的染色质,与10 μl NeuroD (D35G2) Rabbit mAb 兔单抗或者2 μl of Normal Rabbit IgG #2729进行染色质免疫沉淀。使用SimpleChIP® Human EGR1 Promoter Primers #5549、人EGR2启动子引物和SimpleChIP® Human α Satellite Repeat Primers #4486进行real-time PCR定量富集的DNA。每个样本中免疫沉淀的DNA量用总投入量(等于1)的百分比表示。

Background

NeuroD is a member of the basic helix-loop-helix (bHLH) family of transcription factors. These proteins function by forming heterodimers with E-proteins and binding to the canonical E-box sequence CANNTG (1,2). Neuronal activity results in CaMKII-mediated phosphorylation of NeuroD at Ser336, which is necessary for formation and growth of dendrites (3,4). NeuroD is also phosphorylated at Ser274 though the results are context dependent as phosphorylation by Erk stimulates NeuroD activity in pancreatic β-cells while phosphorylation by GSK-3β inhibits NeuroD in neurons (3). NeuroD is crucially important in both the pancreas and developing nervous system, and plays a large role in the development of the inner ear and mammalian retina (3). Mice lacking NeuroD become severely diabetic and die shortly after birth due to defects in β-cell differentiation (2,3,5,6). The lack of NeuroD in the brain results in severe defects in development (5). Human mutations have been linked to a number of types of diabetes including type I diabetes mellitus and maturity-onset diabetes of the young (1,3).

NeuroD是基本的螺旋 - 环 - 螺旋(bHLH)转录因子家族成员。这些蛋白质与E蛋白形成异二聚体,结合经典的E盒序列CANNTG(1,2)。CaMKII介导NeuroD的Ser336磷酸化,这是树突形成和增长所必需的(3,4)。NeuroD的Ser274也可磷酸化,其结果根据磷酸化的酶不同而不同,胰腺β细胞中ERK的磷酸化促进NeuroD活性,神经元中GSK-3β的磷酸化抑制NeuroD活性(3)。NeuroD在胰腺和发育中的神经系统是至关重要的,在内耳和哺乳动物的视网膜的发育中起着很大的作用(3)。缺乏NeuroD的小鼠会发生严重的糖尿病,因缺乏β细胞的分化而在出生不久即死亡(2,3,5,6)。脑内缺乏NeuroD将导致严重的发育障碍(5)。人类该基因突变与I型糖尿病和年轻成年发病的糖尿病有关(1,3)。

  1. Schonhoff, S.E. et al. (2004) Endocrinology 145, 2639-2644.
  2. Sharma, A. et al. (1999) Mol. Cell Biol. 19, 704-713.
  3. Chae, J.H. et al. (2004) Mol. Cells 18, 271-288.
  4. Gaudillière, B. et al. (2004) Neuron 41, 229-241.
  5. Miyata, T. et al. (1999) Genes Dev. 13, 1647-1652.
  6. Naya, F.J. et al. (1997) Genes Dev. 11, 2323-2334.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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