Cell Signaling Technology

Product Pathways - Apoptosis

Phospho-Bad (Ser136) (D25H8) Rabbit mAb #4366

No. Size Price
4366S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4366T 20 µl ( 2 western blots ) ¥1,300.00 现货查询 购买询价
4366 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Monkey, Endogenous 23 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Homology

Species predicted to react based on 100% sequence homology: Rat,

Specificity / Sensitivity

Phospho-Bad (Ser136) (D25H8) Rabbit mAb detects endogenous levels of Bad only when phosphorylated at Ser136 (equivalent to Ser99 in human and Ser137 in rat).

Phospho-Bad (Ser136) (D25H8) Rabbit mAb 兔单抗只能检测内源的Ser136位点 (人的Ser99位点和大鼠的Ser137位点)磷酸化的Bad蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser136 of mouse Bad protein.

该单克隆抗体是采用合成的小鼠Bad Ser136位点周围残基相对应的肽段免疫动物而获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from ACHN cells, untreated or treated with Human Epidermal Growth Factor #8916 (100 ng/ml, 30 min), and C2C12 cells, untreated or insulin-treated (100 nM, 30 min), using Phospho-Bad (Ser136) (D25H8) Rabbit mAb (upper) or Bad (D24A9) Rabbit mAb #9239 (lower).

Western blot免疫印迹分析未处理和经Human Epidermal Growth Factor #8916 (100 ng/ml, 30 min)处理的 ACHN 细胞的细胞提取液,未处理和经insulin处理(100 nM, 30 min)的C2C12细胞的细胞提取液,用Phospho-Bad (Ser136) (D25H8) Rabbit mAb 兔单抗 (上图)或者Bad (D24A9) Rabbit mAb 兔单抗#9239 (下图)研究。

Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7 cells, treated with Human Epidermal Growth Factor #8916 (100 ng/ml, 30 min) in the presence or absence of calf intestinal phosphatase (CIP) plus lambda-phosphatase, using Phospho-Bad (Ser136) (D25H8) Rabbit mAb (upper) or Bad (D24A9) Rabbit mAb #9239 (lower).

Western blot免疫印迹分析细胞裂解液,在小牛肠碱性磷酸酶(CIP)和 lambda 磷酸酶存在或者不存在下,用Human Epidermal Growth Factor #8916 (100 ng/ml, 30 min)处理COS-7细胞, 所用抗体为Phospho-Bad (Ser136) (D25H8) Rabbit mAb 兔单抗(上图)或者Bad (D24A9) Rabbit mAb 兔单抗#9239 (下图)。

Western Blotting

Western Blotting

Western blot analysis of lysates containing Bad Control Proteins #9293, comprised of nonphosphorylated and phosphorylated Bad peptides, using Phospho-Bad (Ser136) (D25H8) Rabbit mAb.

Western blot免疫印迹分析包含Bad Control Proteins #9293的裂解液,包含磷酸化和非磷酸化的Bad蛋白,所用抗体为 Phospho-Bad (Ser136) (D25H8) Rabbit mAb 兔单抗。

Background

Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL (1,2). Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (2). Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xl (2). Akt phosphorylates Bad at Ser136 to promote cell survival (3,4). Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK (5,6) and mitochondria-anchored PKA (7). Phosphorylation of Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL (8-10).

Bad是Bcl-2促凋亡家族中的成员,它通过转移 Bax 与 Bcl-2 和 Bcl-xL 结合而促进细胞死亡(1,2)。存活因子如IL-3, 能够通过激活细胞内信号通路,导致Bad在Ser112和Ser136位点发生磷酸化,进而抑制凋亡活性(2)。在这些位点的磷酸化促进Bad与 14-3-3 蛋白的结合,从而阻止了Bad与Bcl-2和Bcl-xl的互作(2)。Akt在Ser136位点磷酸化Bad,而促进细胞的存活(3,4)。Bad在体内和体外都能够被p90RSK(5,6)和线粒体锚定PKA磷酸化Ser112位点(7)。PKA对BH3结构域Ser155位点的磷酸化,在阻止Bad和Bcl-xL的二聚化过程中发挥了重要的作用(8-10)。

  1. Yang, E. et al. (1995) Cell 80, 285-291.
  2. Zha, J. et al. (1996) Cell 87, 619-628.
  3. Datta, S.R. et al. (1997) Cell 91, 231-241.
  4. Peso, L. et al. (1997) Science 278, 687-689.
  5. Bonni, A. et al. (1999) Science 286, 1358-1362.
  6. Tan, Y. et al. (1999) J. Biol. Chem. 274, 34859-34867.
  7. Harada, H. et al. (1999) Mol. Cell 3, 413-422.
  8. Tan, Y. et al. (2000) J. Biol. Chem. 275, 25865-25869.
  9. Lizcano, J. et al. (2000) Biochem. J. 349, 547-557.
  10. Datta, S. et al. (2000) Mol. Cell 6, 41-51.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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