Cell Signaling Technology

Product Pathways - Metabolism

Phospho-ATP-Citrate Lyase (Ser455) Antibody #4331

ACLY   ATP-citrate   ATP-citrate-pro-S-lyase   ATP-citrate-synthase   citrate-cleavage-enzyme  

No. Size Price
4331S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4331T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
4331 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 125 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,


Species predicted to react based on 100% sequence homology: Rat,

Specificity / Sensitivity

Phospho-ATP-Citrate Lyase (Ser455) Antibody detects endogenous levels of ATP-citrate lyase only when phosphorylated at serine 455.

Phospho-ATP-Citrate Lyase (Ser455) Antibody用于检测内源性在Ser455上磷酸化的ATP-citrate lyase总蛋白水平

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser455 of human ATP-citrate lyase. Antibodies are purified by protein A and peptide affinity chromatography.

多克隆抗体通过用对应于人源ATP-citrate lyase蛋白Ser455序列的合成肽免疫动物制备而得,抗体经蛋白A和肽段亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated or PDGF-treated for the indicated times, using Phospho-ATP-Citrate Lyase (Ser454) Antibody (upper) or ATP-Citrate Lyase Antibody #4332 (lower).

Wetern blot检测NIH/3T3 提取物,未处理或用PDGF处理,所用抗体为Phospho-ATP-Citrate Lyase (Ser455) Antibody (上)和 ATP-Citrate Lyase Antibody #4332 (下)。


ATP-citrate lyase (ACL) is a homotetramer that catalyzes the formation of acetyl-CoA and oxaloacetate (OAA) in the cytosol, which is the key step for the biosynthesis of fatty acids, cholestrol and acetylcholine, as well as for glucogenesis (1). Nutrients and hormones regulate the expression level and phosphorylation of ATP-citrate lyase (1,2). It is phosphorylated by GSK-3 on Thr446 and Ser450 (3). Ser455 of ATP-citrate lyase has been reported to be phosphorylated by PKA and Akt (4,5). Phosphorylation on Ser455 abolishes the homotropic allosteric regulation by citrate and enhances the catalytic activity of the enzyme (2).

ATP-柠檬酸裂解酶(ACL)是一个同源四聚体蛋白,负责在胞浆中催化生成脂酰-CoA和草酰乙酸(OAA),这是脂肪酸、胆固醇和乙醇胆碱生物合成中及糖异生过程的关键步(1)。营养水平和激素调节ATP-柠檬酸裂解酶的表达和磷酸化水平(1,2)。它可以被GsK-3在Thr446 和Ser450磷酸化(3),它的455位Ser可以被PKA和Akt磷酸化(4,5)。Ser455的磷酸化会解除柠檬酸对其的正合别构调节,并加强该酶的催化活性(2)。

  1. Towle, H.C. et al. (1997) Annu Rev Nutr 17, 405-33.
  2. Potapova, I.A. et al. (2000) Biochemistry 39, 1169-79.
  3. Hughes, K. et al. (1992) Biochem J 288 ( Pt 1), 309-14.
  4. Pierce, M.W. et al. (1982) J Biol Chem 257, 10681-6.
  5. Berwick, D.C. et al. (2002) J Biol Chem 277, 33895-900.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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