Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

Phospho-AML1 (Ser249) Antibody #4327

CBF   CBFA2   PEBP2alphaB   Runx1  

No. Size Price
4327S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4327 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 55 Rabbit
IP 1:50
F 1:50
IF-IC 1:800

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Phospho-AML1 (Ser249) Antibody detects endogenous levels of AML1 protein only when phosphorylated on Ser249.

Phospho-AML1 (Ser249)抗体能够检测内源性Ser249位点磷酸化的AML1蛋白水平。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids around Ser249 of human AML1. Antibodies are purified by protein A and peptide affinity chromatography.

此多克隆抗体是通过合成人源对应的AML1 Ser249位点周围的肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts of TPA treated HEL cells, untreated or treated with λ phosphatase, using Phospho-AML1 (Ser249) Antibody (upper), or AML1 Antibody #4334 (lower).Western免疫印迹。用Phospho-AML1 (Ser249) Antibody (上图)或AML1 Antibody #4334 (下图)研究未经处理的和经λ phosphatase处理的TPA处理过的HEL细胞的细胞提取液。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, untreated (green) or treated with λ phosphatase (blue), using Phospho-AML1 (Ser249 ) Antibody compared to a nonspecific negative control antibody (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, untreated or treated with λ phosphatase, using Phospho-AML1 (Ser249) Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析未经处理(绿色) 或 经λ phosphatase处理的HeLa细胞,所用抗体为Phospho-AML1 (Ser249 ) Antibody (绿色),肌丝蛋白用DY-554 phalloidin标记(红色)。Blue pseudocolor = DRAQ5® #4084 (DNA 荧光染料)

Background

AML1 (also known as Runx1, CBFA2, and PEBP2αB) is a member of the core binding factor (CBF) family of transcription factors (1,2). It is required for normal development of all hematopoietic lineages (3-5). AML1 forms a heterodimeric DNA binding complex with its partner protein CBFβ and regulates the expression of cellular genes by binding to promoter and enhancer elements. AML1 is commonly translocated in hematopoietic cancers: chromosomal translocations include t(8;21) AML1-ETO, t(12;21) TEL-AML and t(8;21) AML-M2 (6). Phosphorylation of AML1 on several potential serine and threonine sites, including Ser249, is thought to occur in an Erk-dependent manner (7,8).

AML1 (也叫做Runx1, CBFA2 或 PEBP2αB)是转录因子核心结合因子(CBF)家族中的一员(1,2)。它对造血细胞系的正常发育是不可缺少的(3-5)。AML1与其伴侣蛋白CBFβ形成异二聚体DNA结合复合体,并通过结合启动子和增强子原件而调控细胞基因的表达。AML1 在造血相关癌症中通常发生染色体易位,易位的位置包括t(8;21) AML1-ETO、t(12;21) TEL-AML 和 t(8;21) AML-M2 (6)。磷酸化AML1的几个潜在丝氨酸和苏氨酸位点包括Ser249, 被认为是通过Erk依赖的方式而发生修饰 (7,8)。

  1. Wang, S. et al. (1993) Mol Cell Biol 13, 3324-39.
  2. Ogawa, E. et al. (1993) Proc Natl Acad Sci U S A 90, 6859-63.
  3. Okuda, T. et al. (1996) Cell 84, 321-30.
  4. Wang, Q. et al. (1996) Proc Natl Acad Sci U S A 93, 3444-9.
  5. North, T.E. et al. (2004) Stem Cells 22, 158-68.
  6. Blyth, K. et al. (2005) Nat Rev Cancer 5, 376-87.
  7. Tanaka, T. et al. (1996) Mol Cell Biol 16, 3967-79.
  8. Zhang, Y. et al. (2004) J Biol Chem 279, 53116-25.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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