Cell Signaling Technology

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Angiomotin (D2O4H) Rabbit mAb #43130

No. Size Price
43130S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
43130 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 80, 140 Rabbit IgG
IP 1:100
IF-IC 1:1000

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Angiomotin (D2O4H) Rabbit mAb recognizes endogenous levels of total Angiomotin protein. Based on the sequence of the immunogenic peptide, this antibody is not expected to cross-react with the AMOT family members AMOTL1 or AMOTL2.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near Pro1049 of human Angiomotin protein.

IF-IC

IF-IC

Confocal immunofluorescent analysis of 293T (left) or MCF7 cells (right), using Angiomotin (D2O4H) Rabbit mAb (green). Actin filaments were labeled with DyLightTM 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IP

IP

Immunoprecipitation of Angiomotin from 293T cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Angiomotin (D2O4H) Rabbit mAb. Western blot analysis was performed using Angiomotin (D2O4H) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T and MCF7 cells, using Angiomotin (D2O4H) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human Angiomotin p80 (hAngiomotin p80-Myc/DDK; +) protein, using Angiomotin (D2O4H) Rabbit mAb (upper) or Myc-Tag (71D10) Rabbit mAb #2278 (lower).

Background

Angiomotin (AMOT) is a widely expressed cell junction protein initially identified through its ability to bind angiostatin. Alternative splicing results in two isoforms of angiomotin, the full-length p130 and the amino-terminally truncated p80. These isoforms, along with angiomotin-like 1 (AmotL1) and angiomotin-like 2 (AmotL2) comprise the Motin protein family. Angiomotin is important in endothelial cell polarity, migration and blood vessel formation during development, as well as in signaling through small GTPases and the Hippo/YAP pathway (reviewed in 1).

Research studies have shown that angiomotin expression regulates migration and proliferation of breast cancer cells (2,3).

  1. Moleirinho, S. et al. (2014) FEBS Lett 588, 2693-703.
  2. Zhang, H. and Fan, Q. (2015) Oncol Rep 34, 2163-70.
  3. Lv, M. et al. (2015) Oncol Rep 33, 1938-46.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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