Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

Phospho-p40phox (Thr154) Antibody #4311

No. Size Price
4311S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4311 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 40 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-p40phox (Thr154) Antibody detects endogenous levels of p40phox only when phosphorylated at threonine 154. This antibody does not cross-react with other phosphorylated phox subunits.

Phospho-p40phox (Thr154) 抗体只能检测内源的在thr154位点磷酸化的p40phox蛋白。此抗体不与其它磷酸化的phox亚单元交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr154 of human p40phox. Antibodies are purified by protein A and peptide affinity chromatography.

此多克隆抗体是通过合成人源对应的p40phox Thr154位点周围的磷肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from THP1 cells, untreated or PMA-treated, and guinea pig neutrophils, untreated or fMLP-treated, using Phospho-p40phox (Thr154) Antibody.Western免疫印迹。用Phospho-p40phox (Thr154) Antibody研究未经处理的和经PMA处理的THP1细胞的细胞提取液和未经处理和经fMLP处理的豚鼠噬中性粒细胞的细胞提取液。

Background

The phagocytic NADPH oxidase is a multiprotein enzyme that catalyzes the reduction of oxygen to superoxide in response to invasion of pathogens into the body. The NADPH oxidase consists of 6 subunits, the membrane-bound p91phox and p22phox heterodimer (also known as cytochrome b558), the cytoplasmic complex of p40phox, p47phox and p67phox, and the small GTPase Rac2. Activation of NADPH oxidase is initiated by phosphorylation of the cytosolic complex, which induces comformational changes of the complex and ultimately leads to the translocation of the cytoplasmic complex to the membrane to form an active enzyme with cytochrome b558 (1). Thr154 and Ser315 of p40 phox have been identified as PKC phosphorylation sites modified during activation of the phagocyte NADPH oxidase (2).

噬菌细胞的NADPH氧化酶是多蛋白复合酶,在病原菌侵入时它能催化还原氧成为超氧化物。NADPH 氧化酶包含6个亚单元, 包括膜结合 p91 phox和 p22 phox 异二聚体 (也叫做细胞色素 b558), 细胞质复合体p40phox, p47phox 和 p67phox, 还有小GTPase Rac2。NADPH氧化酶的激活是从细胞质复合体的磷酸化开始, 这就诱导了构象的变化,并导致了细胞质复合体定位到膜上,从而形成具有活性的与细胞色素b558结合的酶 (1)。p40 phox的Thr154 和 Ser315位点被鉴定在激活噬菌细胞NADPH氧化酶是被PKC磷酸化修饰(2)。

  1. Babior, B.M. (1999) Blood 93, 1464-1476.
  2. Bouin, A.P. et al. (1998) J Biol Chem 273, 30097-103.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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