Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

Tuberin/TSC2 (D93F12) XP® Rabbit mAb #4308

hamartin   sc-893   TSC  

No. Size Price
4308S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
4308T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价
4308 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Hamster,Monkey, Endogenous 200 Rabbit IgG
IP 1:50
F 1:6400
IF-IC 1:800

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Tuberin/TSC2 (D93F12) XP® Rabbit mAb detects endogenous levels of total tuberin protein.

Tuberin/TSC2 (D93F12) XP™ Rabbit mAb兔单抗检测内源性的tuberin蛋白的总量。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues of human tuberin protein.

该单克隆抗体是采用与tuberin蛋白羧基末端残基相对应的合成肽段免疫动物生产的。

IF-IC

IF-IC

Confocal immunofluorescent analysis of SH-SY5Y (left), wild-type MEF (middle) and TSC2-deficient (-/-) MEF cells (right) using Tuberin/TSC2 (D93F12) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

共聚焦免疫荧光分析.SH-SY5Y(上图),野生型MEF(中图)和TSC2缺陷型(-/-)MEF细胞(下图),所使用的是Tuberin/TSC2 (D93F12) XP™ Rabbit mAb兔单抗(绿色)。肌动蛋白丝采用DY-554 phalloidin (red)标记。蓝色伪彩为DRAQ5® #4084 (荧光DNA 染料).

Western Blotting

Western Blotting

Western blot analysis of extracts from SH-SY5Y cells and rat brain using Tuberin/TSC2 (D93F12) XP® Rabbit mAb.

Western blot 分析SH-SY5Y洗白和大鼠脑细胞,所用抗体为Tuberin/TSC2 (D93F12) XP® Rabbit mAb兔单抗。

Western Blotting

Western Blotting

Western blot analysis of extracts from wild-type (wt) and TSC2-deficient (-/-) mouse embryonic fibroblasts (MEF) using Tuberin/TSC2 (D93F12) XP® Rabbit mAb (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower).

Western blot 分析野生型(wt)和TSC2缺陷型(-/-)的小鼠的胚胎成纤维细胞(MEF),所使用的是Tuberin/TSC2 (D93F12) XP™ Rabbit mAb 兔单抗(上图)和Akt (pan) (C67E7) Rabbit mAb兔单抗 #4691 (下图)。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of wild-type MEF (green) and TSC2-deficient (-/-) MEF cells (blue) using Tuberin/TSC2 (D93F12) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of wild-type MEF (green) and TSC2-deficient (-/-) cells (blue) using Tuberin/TSC2 (D93F12) XP® Rabbit mAb (PE Conjugate).

Background

Tuberin is a product of the TSC2 tumor suppressor gene and an important regulator of cell proliferation and tumor development (1). Mutations in either TSC2 or the related TSC1 (hamartin) gene cause tuberous sclerosis complex (TSC), an autosomal dominant disorder characterized by development of multiple, widespread non-malignant tumors (2). Tuberin is directly phosphorylated at Thr1462 by Akt/PKB (3). Phosphorylation at Thr1462 and Tyr1571 regulates tuberin-hamartin complexes and tuberin activity (3-5). In addition, tuberin inhibits the mammalian target of rapamycin (mTOR), which promotes inhibition of p70 S6 kinase, activation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1, an inhibitor of translation initiation), and eventual inhibition of translation (3,6,7).

马铃薯球蛋白(Tuberin)是TSC2肿瘤抑制基因的产物,是细胞增殖和肿瘤发展的重要调节因子(1)。TSC2或与之相关的TSC1(hamartin)的突变可以引起结节硬化复合体TSC(tuberous sclerosis complex),是一种常染色体显性遗传病,其特点为发生多种广泛的非恶性肿瘤(2)。Tuberin可以在Thr1462位点直接被Akt/PKB 磷酸化(3)。 在Thr1462 和 Tyr1571位点的磷酸化可以调节tuberin-hamartin复合体和tuberin的活性(3-5)。另外,tuberin可以抑制mTOR(mammalian target of rapamycin), 进而推动p70 S6激酶的抑制和真核细胞抑制因子4E结合蛋白(4E-BP1,一种转录起始抑制因子)的活性,并最终抑制翻译(3,6,7)。

  1. Soucek, T. et al. (1998) Proc Natl Acad Sci U S A 95, 15653-8.
  2. Sparagana, S.P. and Roach, E.S. (2000) Curr Opin Neurol 13, 115-9.
  3. Manning, B.D. et al. (2002) Mol Cell 10, 151-62.
  4. Aicher, L.D. et al. (2001) J Biol Chem 276, 21017-21.
  5. Dan, H.C. et al. (2002) J Biol Chem 277, 35364-70.
  6. Goncharova, E.A. et al. (2002) J Biol Chem 277, 30958-67.
  7. Inoki, K. et al. (2002) Nat Cell Biol 4, 648-57.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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