Cell Signaling Technology

Product Pathways - Protein Stability

K48-linkage Specific Polyubiquitin Antibody #4289

K-48   K48   K48-linked   K48ub   ub   ubiquitin  

No. Size Price
4289S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4289 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,All Species Expected, Endogenous Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

K48-linkage Specific Polyubiquitin 抗体识别在Lys48链接的多泛素化链。这一抗体与单泛素和其他残基偶联的多泛素链无交叉反应。此抗体显示出与线性多泛素链轻微的交叉反应,与其他赖氨酸残基偶联的单泛素或多泛素链没有交叉反应。

Source / Purification

这一单抗是利用合成对应于人双泛素链的Lys48位分枝序列的肽段免疫动物获得,抗体经Protein A和肽亲和层析纯化。 Western blot analysis of recombinant monoubiquitin, K48-linked polyubiquitin, K48-linked polyubiquitin and K63-linked polyubiquitin using K48-linkage Specific Polyubiquitin Antibody (upper) and Ubiquitin (P4D1) Mouse mAb #3936 (lower). 使用K48-linkage Specific Polyubiquitin抗体(上)和泛素(P4D1)小鼠单克隆抗体#3936(下)检测重组单泛素、K48-偶联多泛素和K63-偶联多泛素的免疫印迹分析结果。 Western blot analysis of seven distinct recombinant polyubiquitin chains using K48-linkage Specific Polyubiquitin Antibody (upper) and Ubiquitin (P4D1) Mouse mAb #3936 (lower). 使用K48-linkage Specific Polyubiquitin抗体(上)和泛素(P4D1)小鼠单克隆抗体#3936(下)对七个不同的重组泛素链进行Western blot分析的结果。 Western blot analysis of L363 cells, untreated or treated with bortezomib as indicated, using K48-linkage Specific Polyubiquitin Antibody. 使用K48-linkage Specific Polyubiquitin抗体对经或未经bortezomib处理的L363细胞裂解物中该蛋白进行免疫印迹分析的结果。

Western Blotting

Western Blotting

Western blot analysis of recombinant monoubiquitin, K48-linked polyubiquitin, K48-linked polyubiquitin and K63-linked polyubiquitin using K48-linkage Specific Polyubiquitin Antibody (upper) and Ubiquitin (P4D1) Mouse mAb #3936 (lower).

Western Blotting

Western Blotting

Western blot analysis of seven distinct recombinant polyubiquitin chains using K48-linkage Specific Polyubiquitin Antibody (upper) and Ubiquitin (P4D1) Mouse mAb #3936 (lower).

Western Blotting

Western Blotting

Western blot analysis of L363 cells, untreated or treated with bortezomib as indicated, using K48-linkage Specific Polyubiquitin Antibody.

Background

Ubiquitin is a conserved polypeptide unit that plays an important role in the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue (1-3). The ubiquitin-proteasome pathway has been implicated in a wide range of normal biological processes and in disease-related abnormalities. Several proteins such as IκB, p53, cdc25A, and Bcl-2 have been shown to be targets for the ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis (4-7).

泛素是一种保守的多肽单元,在泛素蛋白酶体途径中起重要作用。泛素能与许多细胞蛋白共价连接,并被26S蛋白酶降解。目标蛋白泛素共价结合的进程终包含3种组分。首先泛素通过与E1泛素活化酶形成硫酯复合物而被活化。活化的泛素随后被转运到E2泛素载体蛋白,随后E3泛素连接酶把E2连接到靶蛋白的赖氨酸的epsilon位氨基上(1-3)。泛素蛋白酶体通路在正常生物过程和疾病中广泛存在。一些蛋白,诸如IκB, p53, cdc25a, 和Bcl-2等都是泛素-蛋白酶体途径的靶蛋白,并可以调节细胞周期进展、分化、细胞应激反应以及凋亡(4-7)。

在底物蛋白泛素化修饰过程中,最常见的连接位点为泛素的7个lysine残基(Lys6, Lys11, Lys27, Lys29, Lys33, Lys48 和 Lys63)。 多泛素链的形成是通过一个泛素的lysine残基又连接到其他泛素的羧基端。在不同lysine残基链接形成的多泛素化组装介导着不同的功能,K48残基链接的多泛素化主要参与蛋白质的降解,而K63位链接的多泛素化主要调节蛋白质的功能、定位和蛋白质相互作用(8)。

  1. Ciechanover, A. (1998) EMBO J 17, 7151-60.
  2. Hochstrasser, M. (2000) Nat Cell Biol 2, E153-7.
  3. Hochstrasser, M. (2000) Science 289, 563-4.
  4. Bernardi, R. et al. (2000) Oncogene 19, 2447-54.
  5. Aberle, H. et al. (1997) EMBO J 16, 3797-804.
  6. Salomoni, P. and Pandolfi, P.P. (2002) Nat Cell Biol 4, E152-3.
  7. Jesenberger, V. and Jentsch, S. (2002) Nat Rev Mol Cell Biol 3, 112-21.
  8. Komander, D. (2009) Biochem Soc Trans 37, 937-53.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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