Cell Signaling Technology

Product Pathways - Metabolism

Phospho-AS160 (Thr642) Antibody #4288

AS160   TBC1 domain   TBC1 domain family. member 4   TBC1D   TBC1D4   Thr642  

No. Size Price
4288S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4288 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 160 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Mouse, Rat,

Specificity / Sensitivity

Phospho-AS160 (Thr642) Antibody detects endogenous levels of AS160 protein only when phosphorylated at Thr642.

Phospho-AS160 (Thr642) Antibody用于检测内源性在642苏氨酸位点上磷酸化的AS160蛋白水平

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence around Thr642 of human AS160. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from serum starved HeLa cells, untreated or treated with either insulin (150 nM, 15 minutes) or IGF-1 #3093 (100 ng/ml, 15 minutes), using Phospho-AS160 (Thr642) Antibody (upper) or AS160 (C69A7) Rabbit mAb #2670 (lower). The phospho-specificity of the antibody is verified by λ-phosphatase treatment of the above cell lysates.

Wetern blot检测血清饥饿的Hela细胞提取物,未处理或用胰岛素和IGF-1处理,所用抗体为Phospho-AS160 (Thr642) Antibody (上)和AS160 (C69A7) Rabbit mAb兔单抗 #2670 (下)。抗体的磷酸化特异性通过λ磷酸酶处理来验证。


The polypeptide insulin is a major hormone controlling critical energy functions such as glucose and lipid metabolism. Insulin binds to and activates the insulin receptor (IR) tyrosine kinase, which phosphorylates and recruits different adaptor proteins. The signaling pathway initiated by insulin and its receptor stimulates glucose uptake in muscle cells and adipocytes through translocation of GLUT4 glucose transporter from the cytoplasm to the plasma membrane (1). A 160 kDa substrate of the Akt Ser/Thr kinase (AS160, TBC1D4) is a Rab GTPase-activating protein that regulates insulin-stimulated GLUT4 trafficking. AS160 is expressed in many tissues including brain, kidney, liver, and brown and white fat (2). Multiple Akt phosphorylation sites have been identified on AS160 in vivo, with five sites (Ser318, Ser570, Ser588, Thr642, and Thr751) showing increased phosphorylation following insulin treatment (2,3). Studies using recombinant AS160 demonstrate that insulin-stimulated phosphorylation of AS160 is a crucial step in GLUT4 translocation (3) and is reduced in some patients with type 2 diabetes (4). The interaction of 14-3-3 regulatory proteins with AS160 phosphorylated at Thr642 is a necessary step for GLUT4 translocation (5). Phosphorylation of AS160 by AMPK is involved in the regulation of contraction-stimulated GLUT4 translocation (6).

多肽类激素胰岛素是控制能量代谢如糖脂代谢的一个主要激素。胰岛素结合并激活胰岛素受体酪氨酸激酶,受体酪氨酸激酶可以招募并磷酸化各种接头蛋白。在骨胳肌和脂肪细胞中,由胰岛素及其受体引发的信号通路可以增加细胞的糖摄入,这是通过将Glut4葡萄糖转运小体从胞浆转运到质膜上实现的(1)。有一种分子量的160 kDa的AKt丝氨酸/苏氨酸激酶底物(AS160,TBC1D4)是一种Rab GTP酶激活的蛋白质,它可以调节胰岛素介导的Glut4转运。AS160在多种组织中表达,包括大脑,肾脏,肝脏,白色脂肪及棕色脂肪(2)。在体内,已经鉴定出一多个AS160蛋白的AKt激酶磷酸化位点,其中的五个位点(Ser318, Ser570, Ser588, Thr642, 和Thr751),在用胰岛素处理后,磷酸化水平增加(2,3)。一些用重组AS160蛋白的研究表明,胰岛素诱导的AS160磷酸化是GLUT4的转运过程中的一个重要步骤(3),并且在2型糖尿病中,该蛋白的磷酸化水平降低(4)。14-3-3调节蛋白与Thr642磷酸化AS160的结合是GLUT4的转运过程中的一个必需步骤(5)。在浓度诱导的GLUT4的转运中,AMPK对AS160的磷酸化也参与其中。

  1. Watson, R.T. and Pessin, J.E. (2006) Trends Biochem. Sci. 31, 215-222.
  2. Kane, S. et al. (2002) J. Biol. Chem. 277, 22115-22118.
  3. Sano, H. et al. (2003) J. Biol. Chem. 278, 14599-14602.
  4. Karlsson, H.K. et al. (2005) Diabetes 54, 1692-1697.
  5. Ramm, G. et al. (2006) J. Biol. Chem. 281, 29174-29180.
  6. Kramer, H.F. et al. (2006) J. Biol. Chem. 281, 31478-31485.

Application References

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