Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

α-Tubulin (11H10) Rabbit mAb (Biotinylated) #4285

a-tubulin   Alpha-tubulin ubiquitous   K-Alpha-1 alpha tubulin {K-ALPHA-1}   TBAK   Tubulin alpha-ubiquitous chain   Tubulin K-alpha-1  

No. Size Price
4285S 100 µl ( 10 western blots ) ¥3,986.00 现货查询 购买询价
4285 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey,D. melanogaster,Zebrafish,Bovine,Pig, Endogenous 52 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Dog,

Specificity / Sensitivity

α-Tubulin (11H10) Rabbit mAb (Biotinylated) detects endogenous levels of total α-tubulin protein and does not cross-react with recombinant β-tubulin.

α-Tubulin (11H10) Rabbit mAb (Biotinylated)兔单抗检测内源性α-tubulin总蛋白,但是不能与重组的β-tubulin蛋白发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to the amino terminus of human α-tubulin.

通过人工合成人源α-tubulin蛋白氨基端相应的磷酸化片段去免疫动物从而制备出单克隆抗体。

Description

This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated α-Tubulin (11H10) Rabbit mAb #2125.

Cell Signaling Technology抗体在最佳的条件下连接着biotin。该抗体被期望与非连接的α-Tubulin (11H10) Rabbit mAb #2125有相同的物种交叉反应。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using α-Tubulin (11H10) Rabbit mAb (Biotinylated) and developed using Streptavidin-HRP #3999.

使用α-Tubulin (11H10) Rabbit mAb (Biotinylated)以及随后使用Streptavidin-HRP #3999,免疫印迹(Western Blot)分析不同细胞中α-Tubulin蛋白水平。

Background

The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).

细胞骨架由三种类型的细胞质基质纤维组成:微管、微丝(肌动蛋白纤维)和中间纤维。球形微管蛋白亚基包含微管建筑块,由α/β-tubulin异源二聚体形成所有真核细胞的tubulin亚单位。γ-tubulin被需要在tubulin亚单位的成核聚合作用中去形成微管聚合物。许多细胞运动是通过微管动力介导的,包括纤毛和鞭毛的跳动、膜泡的核质转运、在减数分裂/有丝分裂期间染色体排布和神经轴突的迁移。这些运动由于竞争性微管聚合作用和解聚作用或通过微管动力蛋白的运动(1)。

  1. Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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