Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Phospho-Myt1 (Ser83) Antibody #4281

No. Size Price
4281S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4281T 20 µl ( 2 western blots ) ¥1,300.00 现货查询 购买询价
4281 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 70 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-Myt1 (Ser83) Antibody detects endogenous levels of Myt1 only when phosphorylated at serine 83. Phospho-Myt1 (Ser83) Antibody 能够检测内源性丝氨酸(83位)磷酸化的Myt1总蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser83 of human Myt1. Antibodies are purified by protein A and peptide affinity chromatography. 该多克隆抗体是由合成的人源的针对Myt1蛋白丝氨酸(83位)的磷酸化肽段免疫动物,利用蛋白A和多肽亲和层析技术生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts from HT29 cells, untreated or nocodazole-treated (50ng/ml), using Phospho-Myt1 (Ser83) Antibody (upper) or Myt1 Antibody #4282 (lower). western blot方法检测未处理或nocodazole(50ng/ml)处理的HT29细胞提取物,是由的抗体为 Phospho-Myt1 (Ser83) Antibody (上图)或Myt1 Antibody #4282 (下图)。


Entry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. The critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Thr14 (1,2). Phosphorylation at Tyr15 and Thr14 and inhibition of cdc2 is carried out by Wee1 and Myt1 protein kinases, while Tyr15 dephosphorylation and activation of cdc2 is carried out by the cdc25 phosphatase (1,3,4). Hyperphosphorylation and inactivation of Myt1 in mitosis suggests that one or more kinases activated at the G2/M transition negatively regulates Myt1 activity. Kinases shown to phosphorylate Myt1 include cdc2, p90RSK, Akt, and Plk1 (5-8). cdc2激酶的激活参与调节所有的真核细胞进入有丝分裂。进入有丝分裂过程中cdc2激活的关键调节步骤似乎是酪氨酸15位和14位的去磷酸化(1,2)。Wee1和Myt1蛋白激酶可以磷酸化酪氨酸15位和14位并抑制cdc2,而cdc25磷酸酶能够去磷酸化酪氨酸15位并激活cdc2(1,3,4)。有丝分裂中Myt1的过磷酸化和失活表明一个或多个激酶在G2/M转换期激活并负调控Myt1的活性。能够磷酸化Myt1的激酶有cdc2, p90RSK, Akt和Plk1 (5-8)。

Although Akt has been shown to phosphorylate Asterina (starfish) Myt1 at a consensus Akt phosphorylation site (7), the orthologous site, Ser83, in human Myt1 may be phosphorylated by a different kinase. Akt被揭示能够磷酸化海燕(海星)Myt1蛋白一个公认的Akt磷酸化位点(7), 但人类Myt1蛋白的同源位点(丝氨酸83位)的磷酸化却可能是其他激酶的作用。

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Application References

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