Product Pathways - Cell Cycle / Checkpoint
CDK4 (D9G3E) Rabbit mAb (PE Conjugate) #42749
|42749S||100 µl ( 50 tests )||￥3,986.00 现货查询||购买询价|
|42749||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: F=Flow Cytometry,
Specificity / Sensitivity
CDK4 (D9G3E) Rabbit mAb (PE Conjugate) recognizes endogenous levels of total CDK4 protein. In COS-7 cells, this antibody recognizes a 40 kDa background band of unknown origin. This antibody does not cross-react with CDK1, 2, or 6.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CDK4 protein.
This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated CDK4 (D9G3E) Rabbit mAb #12790.
Cyclin-dependent kinase activity is regulated by T-loop phosphorylation (Thr172 in the case of CDK4), by the abundance of their cyclin partners, and by association with CDK inhibitors of the Cip/Kip or INK family of proteins (1). The inactive ternary complex of CDK4/cyclin D and p27 Kip1/Cip1 requires extracellular mitogenic stimuli for the release and degradation of p27, which affects progression through the restriction point and pRb-dependent entry into S-phase (2). The active complex of CDK4/cyclin D targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression (3). In HeLa cells, upon UV irradiation, upregulation of p16 INK4A association with CDK4/cyclin D3 leads to a G2 delay, implicating CDK4/cyclin D3 activity in progression through the G2-phase of the cell cycle (4).
- Hirai, H. et al. (1995) Mol Cell Biol 15, 2672-81.
- Sherr, C.J. (1996) Science 274, 1672-7.
- Lukas, J. et al. (1996) Mol Cell Biol 16, 6917-25.
- Gabrielli, B.G. et al. (1999) J Biol Chem 274, 13961-9.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
For Research Use Only. Not For Use In Diagnostic Procedures.
DRAQ5 is a registered trademark of Biostatus Limited.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
用户评论 --- 共 0 条