Cell Signaling Technology

Product Pathways - Metabolism

THEM2 Antibody #42713

ACO13   ACOT13   Acyl-CoA thioesterase 13   Acyl-coenzyme A thioesterase 13   HT012   hypothalamus protein HT012   MGC4961   PNAS-27   THEM2   Thioesterase superfamily member 2  

No. Size Price
42713S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
42713 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 15 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

THEM2 Antibody recognizes endogenous levels of total THEM2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human THEM2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using THEM2 Antibody.

IP

IP

Immunoprecipitation of THEM2 from COLO 205 cell extracts. Lane 1 is 10% input, lane 2 is Normal Rabbit IgG #2729, and lane 3 is THEM2 Antibody. Western blot analysis was performed using THEM2 Antibody.

Background

THEM2 is a homotetrameric fatty acyl–CoA thioesterase (1). THEM2 and PC-TP (phosphatidylcholine transfer protein), both enriched in liver, interact to form a complex (1). Cell membrane-bound phosphatidylcholines bind to PC-TP in the complex (1). The complex in turn inhibits IRS-2 and mTORC1, which leads to the suppression of insulin signaling (1). THEM2 has also been shown to regulate adaptive thermogenesis in mice (2).

  1. Ersoy, B.A. et al. (2013) Sci Signal 6, ra64.
  2. Kang, H.W. et al. (2013) J Biol Chem 288, 33376-86.

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

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