Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-SRF (Ser103) Antibody #4261

No. Size Price
4261S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4261 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 67 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-SRF (Ser103) Antibody detects endogenous levels of SRF only when phosphorylated at serine 103. This antibody cross-reacts with a non-specific protein of approximately 52 kDa.

Phospho-SRF (Ser103) Antibody 兔多抗能检测内源性丝氨酸(103位点)磷酸化的SRF蛋白水平。该抗体能与大约52 kDa的非特异性蛋白发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around serine 103 of human SRF. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体通过使用与人源SRF蛋白丝氨酸(103位点)周围残基相一致的合成肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 and HeLa cells untreated or TPA-treated, using Phospho-SRF (Ser103) Antibody.western blot方法检测细胞提取物:未经处理和TPA处理的NIH/3T3、HeLa细胞,使用的抗体是Phospho-SRF (Ser103) Antibody。

Background

The serum response factor (SRF) is a 67 kDa phospho-protein that, together with auxiliary factors, modulates transcription of immediate early genes containing serum response elements at their promoters (1,2). SRF contains several phosphorylation sites (3), but functional consequences of phosphorylation have not been identified unequivocally. Several growth factor- and calcium-regulated kinases, such as p90RSK and CaM kinase IV, can phosphorylate SRF at Ser103 (4,5), and Ser103 of SRF is also a nuclear target for MAPKAP kinase 2 (6).

血清效应因子(SRF) 是67 kDa的磷酸化蛋白,它与辅助因子一起修饰即时早期基因的转录,这些基因的启动子中包含血清效应元件(1,2)。SRF包含一些磷酸化位点(3),但是磷酸化的功能性序列目前还不明确。一些生长因子和钙调节激酶,如p90RSK、CaM kinase IV,能磷酸化SRF蛋白的丝氨酸103位点(4,5),SRF蛋白的丝氨酸103位点是MAPKAP激酶2的细胞核上靶点(6)。

  1. Treisman, R. (1995) EMBO J. 14, 4905-4913.
  2. Janknecht, R. et al. (1995) Carcinogenesis 16, 443-450.
  3. Marais, R. M. et al. (1992) EMBO J. 11, 97-105.
  4. Rivera, V. M. et al. (1993) Mol. Cell. Biol. 13, 6260-6273.
  5. Miranti, C. K. et al. (1995) Mol. Cell. Biol. 15, 3672-3684.
  6. Heidenreich, O. et al. (1999) JBC 274, 14434-14443.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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