Cell Signaling Technology

Product Pathways - Apoptosis

DR4 (D9S1R) Rabbit mAb #42533

No. Size Price
42533S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
42533 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 35-55 Rabbit IgG
IP 1:100
F 1:50
IF-IC 1:800

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

DR4 (D9S1R) Rabbit mAb recognizes endogenous levels of total DR4 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminal, cytoplasmic domain of human DR4 protein.



Confocal immunofluorescent analysis of HCT 116 (positive, left) and FaDu (negative, right) cells using DR4 (D9S1R) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of U266 cells using DR4 (D9S1R) Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype control #3900 (red). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length human DR4 (hDR4; +) using DR4 (D9S1R) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using DR4 (D9S1R) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).



Immunoprecipitation of DR4 from PC-3 cell extracts. Lane 1 represents 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is DR4 (D9S1R) Rabbit mAb. Western blot was performed using DR4 (D9S1R) Rabbit mAb. A conformation specfic secondary antibody was used to avoid IgG cross-reactivity.


The tumor necrosis factor receptor family, which includes TNF-RI, Fas, DR3, DR4, DR5, and DR6, plays an important role in the regulation of apoptosis in various physiological systems (1,2). The receptors are activated by a family of cytokines that include TNF, FasL, and TRAIL. They are characterized by a highly conserved extracellular region containing cysteine-rich repeats and a conserved intracellular region of about 80 amino acids termed the death domain (DD). The DD is important for transducing the death signal by recruiting other DD containing adaptor proteins (FADD, TRADD, RIP) to the death-inducing signaling complex (DISC), resulting in activation of caspases.

DR4 (TRAIL-RI, TNFRSF10A) and DR5 (TRAIL-R2, TNFRSF10B) are receptors for the cytokine TRAIL. Both receptors contain death domains that recruit DISC complexes triggering caspase activation and apoptosis (3-6). The ability of TRAIL to selectively kill malignant cells has led to clinical studies involving TRAIL and receptor agonists (7).

  1. Nagata, S. (1997) Cell 88, 355-65.
  2. Thorburn, A. (2004) Cell Signal 16, 139-44.
  3. Pan, G. et al. (1997) Science 276, 111-3.
  4. Walczak, H. et al. (1997) EMBO J 16, 5386-97.
  5. Chaudhary, P.M. et al. (1997) Immunity 7, 821-30.
  6. Schneider, P. et al. (1997) Immunity 7, 831-6.
  7. Yang, A. et al. (2010) Curr Opin Cell Biol 22, 837-44.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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