Cell Signaling Technology

Product Pathways - Neuroscience

FEZ1 (D9R8Q) Rabbit mAb #42480

No. Size Price
42480S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
42480 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 65 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

FEZ1 (D9R8Q) Rabbit mAb recognizes endogenous levels of total FEZ1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human FEZ1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human FEZ1 (hFEZ1-Myc/DDK; +), using FEZ1 (D9R8Q) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from human cortex, mouse brain, and rat brain using FEZ1 (D9R8Q) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using FEZ1 (D9R8Q) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from Malme-3M cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® FEZ1 siRNA I #72660. (+), using FEZ1 (D9R8Q) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The FEZ1 (D9R8Q) Rabbit mAb confirms silencing of FEZ1 expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control.

Background

The coiled-coil containing protein fasciculation and elongation protein zeta-1 (FEZ1) is expressed predominately in the brain and is the mammalian ortholog of the C. elegans protein UNC-76. It was identified independently in several interaction screens using distinct baits and was shown to play a role in neuronal differentiation and outgrowth, viral defense, centrosome organization, cytoskeletal signaling, and autophagy (reviewed in 1). It was originally identified as a binding partner and substrate for PKCζ and was found to induce the neuronal differentiation of PC-12 cells when co-expressed with active PKCζ (2). FEZ1 was also found to be an interacting partner with the schizophrenia-associated protein DISC1, which may suggest a role for FEZ1 in schizophrenia as well as other mental disorders (3,4). FEZ1 has also been shown to bind to several cytoskeletal proteins, including kinesins, tubulins, JIP1, NEK1, and CLASP2, which supports its role in neurite outgrowth, cargo transport along microtubules, and centrosomal organization (5-7). Additional research studies have shown that FEZ1 interacts with a viral agnoprotein and plays a role in viral defense, including during HIV-1 infection (8-10). Another screen identified FEZ1 as a binding partner for the ubiquitin ligase E4B and showed that FEZ1 can be regulated through polyubiquitination (11). Moreover, degradation of FEZ1 by the ubiquitination-proteasomal pathway through cdc20 provides a mechanism for FEZ1 in dendritic outgrowth (12). FEZ1 was also found to regulate autophagy through association with ULK1 and Beclin-1 complexes (13).

  1. Maturana, A.D. et al. (2010) ScientificWorldJournal 10, 1646-54.
  2. Kuroda, S. et al. (1999) J Cell Biol 144, 403-11.
  3. Miyoshi, K. et al. (2003) Mol Psychiatry 8, 685-94.
  4. Kang, E. et al. (2011) Neuron 72, 559-71.
  5. Fujita, T. et al. (2007) Biochem Biophys Res Commun 361, 605-10.
  6. Blasius, T.L. et al. (2007) J Cell Biol 176, 11-7.
  7. Lanza, D.C. et al. (2010) Mol Cell Biochem 338, 35-45.
  8. Suzuki, T. et al. (2005) J Biol Chem 280, 24948-56.
  9. Naghavi, M.H. et al. (2005) Genes Dev 19, 1105-15.
  10. Haedicke, J. et al. (2009) Proc Natl Acad Sci U S A 106, 14040-5.
  11. Okumura, F. et al. (2004) J Biol Chem 279, 53533-43.
  12. Watanabe, Y. et al. (2014) Cell Rep 7, 552-64.
  13. McKnight, N.C. et al. (2012) EMBO J 31, 1931-46.

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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