Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

CHD4 Antibody #4245

ATP-dependent helicase CHD4   Mi-2   Mi-2 autoantigen 218 kDa protein   Mi2   Mi2-b   Mi2-beta   nurd complex  

No. Size Price
4245S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
4245 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, 260 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Monkey, Bovine, Horse,

Specificity / Sensitivity

CHD4 Antibody detects endogenous levels of total CHD4 protein.

CHD4 Antibody能够检测内源性的CHD4总蛋白水平。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human CHD4 protein. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from 293 and NCCIT cells using CHD4 Antibody.

使用CHD4 Antibody,免疫印迹(Western blot)分析293和NCCIT细胞中CHD4蛋白水平。


Chromodomain-helicase-DNA-binding domain (CHD) proteins have been identified in a variety of organisms (1,2). This family of nine proteins is divided into three separate subfamilies: subfamily I (CHD1 and CHD2), subfamily II (CHD3 and CHD4), and subfamily III (CHD5, CHD6, CHD7, CHD8, CHD9). All CHD proteins contain two tandem amino-terminal chromodomains, a SWI/SNF-related ATPase domain, and a carboxy-terminal DNA binding domain (1,2). The chromodomains facilitate binding to methylated lysine residues of histone proteins and confer interactions with specific regions of chromatin. The SWI/SNF-related ATPase domain utilizes energy from ATP hydrolysis to modify chromatin structure. CHD proteins are often found in large, multi-protein complexes, with their transcriptional activation or repression activity governed by other proteins also found in the complex. CHD3 (also known as Mi2-α) and CHD4 (also known as Mi2-β) are central components of the nucleosome remodeling and histone deacetylase (NuRD) transcriptional repressor complex, which also contains HDAC1, HDAC2, RbAp48, RbAp46, MTA1, MTA2, MTA3, and MBD3 (3-8). Both CHD3 and CHD4 contain two PHD (plant homeodomain) zinc finger domains that bind directly to HDAC1 and HDAC2.

Chromodomain-helicase-DNA-binding domain (CHD)蛋白已经在多种生物中被鉴定(1,2)。由九个成员组成的蛋白家族已经被分为三个亚家族:subfamily I (CHD1和CHD2)、subfamily II (CHD3和CHD4)和subfamily III (CHD5、CHD6、CHD7、CHD8、CHD9)。所有CHD蛋白包含两个串联的N端chromodomains、一个SWI/SNF相关的ATPase结构域和一个C端DNA结合区域 (1,2)。chromodomains有助于结合到组蛋白的已甲基化赖氨酸残基,并且可与染色质的特异区域相互作用。SWI/SNF相关的ATPase区域可利用来自ATP水解的能量去修饰染色质结构。CHD蛋白经常发现在大的多蛋白复合物中,伴随着转录激活或抑制活性,这有该复合物中其它蛋白质监管。CHD3 (也称为Mi2-α)和CHD4 (也称为Mi2-β)是核小体重塑和组蛋白去乙酰化酶(NuRD)转录抑制复合物的中心成员,其包含HDAC1、HDAC2、RbAp48、RbAp46、MTA1、MTA2、MTA3和MBD3 (3-8)。CHD3和CHD4包含两个PHD (plant homeodomain)锌指结构,该结构直接结合到HDAC1和HDAC2。

  1. Hall, J.A. and Georgel, P.T. (2007) Biochem Cell Biol 85, 463-76.
  2. Marfella, C.G. and Imbalzano, A.N. (2007) Mutat Res 618, 30-40.
  3. Tong, J.K. et al. (1998) Nature 395, 917-21.
  4. Xue, Y. et al. (1998) Mol Cell 2, 851-61.
  5. Zhang, Y. et al. (1998) Cell 95, 279-89.
  6. Bowen, N.J. et al. (2004) Biochim Biophys Acta 1677, 52-7.
  7. Jones, P.L. et al. (1998) Nat Genet 19, 187-91.
  8. Fujita, N. et al. (2003) Cell 113, 207-19.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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