Cell Signaling Technology

Product Pathways - Metabolism

Tom20 (D8T4N) Rabbit mAb #42406

sc-11415   sc-17764  

No. Size Price
42406S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
42406 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 16 Rabbit IgG
IP 1:50
IF-IC 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Tom20 (D8T4N) Rabbit mAb recognizes endogenous levels of total Tom20 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Tom20 protein.



Confocal immunofluorescent analysis of HeLa (high expression, left) and Saos-2 (low expression, right) cells, using Tom20 (D8T4N) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and Saos-2 cells using Tom20 (D8T4N) Rabbit mAb (upper) and β-Tubulin (D2N5G) Rabbit mAb #15115 (lower).


Mitochondria play a central role in cellular energy metabolism and are essential organelles in eukaryotes. In humans, 13 proteins are encoded by the mitochondrial genome while the vast majority of mitochondrial proteins are encoded by the nuclear genome. As a result, most mitochondrial proteins are synthesized as precursors in the cytoplasm and imported across mitochondrial membranes by one or more translocase protein complexes (1). The translocase of the outer mitochondrial membrane (TOM complex) facilitates the import of proteins through the outer mitochondrial membrane, while the complementary translocase of the inner membrane (TIM complex) is responsible for protein transport to the mitochondrial matrix. The TOM complex consists of the receptors Tom20, Tom22, and Tom70, and the channel-forming protein Tom40 (1). Tom20 is localized in the outer mitochondrial membrane and initially recognizes precursors with a presequence to facilitate protein import across the outer mitochondrial membrane (2). In a sequential process, recognition of the presequence by Tom20 is followed by tethering of the presequence to the Tom40 protein complex for efficient protein import (3).

  1. Chacinska, A. et al. (2009) Cell 138, 628-44.
  2. Saitoh, T. et al. (2007) EMBO J 26, 4777-87.
  3. Yamamoto, H. et al. (2011) Proc Natl Acad Sci U S A 108, 91-6.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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