Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb (Sepharose® Bead Conjugate) #4232

bead conjugate   H3   H3K27   H3K27me3   Histone  

No. Size Price
4232S 400 µl ( 40 immunoprecipitations ) ¥3,986.00 现货查询 购买询价
4232 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
IP 1:20 Human,Mouse,Rat,Monkey, Endogenous 17 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: IP=Immunoprecipitation,

Homology

Species predicted to react based on 100% sequence homology: Xenopus, Zebrafish,

Specificity / Sensitivity

Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb (Sepharose Bead Conjugate) recognizes endogenous levels of histone H3 only when tri-methylated on Lys27. The antibody does not cross-react with non-methylated, mono-methylated, or di-methylated Lys27. In addition, the antibody does not cross-react with mono-methylated, di-methylated, or tri-methylated histone H3 at Lys4, Lys9, Lys36, or Histone H4 at Lys20.

Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb兔单抗(Sepharose Bead Conjugate)能够检测内源性的Lys27位点三甲基化的组蛋白H3。该抗体不会与Lys27位点非甲基化,单甲基化或双甲基化组蛋白H3发生交叉反应。此外,也不会与Lys4,Lys9,Lys36 位点单甲基化,二甲基化或三甲基化的组蛋白H3或Lys20位点单、二或三甲基化的组蛋白H4发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of histone H3 in which Lys27 is tri-methylated.

该单克隆抗体是经由合成的Lys27位点三甲基化组蛋白H3的氨基端肽段免疫动物而生产的。

Description

This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb (Sepharose Bead Conjugate) is useful for the immunoprecipitation of tri-methyl-histone H3 (Lys27). The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733.

该CST抗体是通过共价结合伯胺基团到NHS活化的琼脂糖珠上来进行固定的。Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb兔单抗(Sepharose Bead Conjugate)对于三甲基化组蛋白H3(Lys27)的免疫沉淀非常有用。它与未偶联的Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733具有相同的种属交叉反应性。

IP

IP

Immunoprecipitation of tri-methyl-histone H3 (Lys27) from HeLa cell extracts using Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb (Sepharose Bead Conjugate) and Rabbit (DA1E) mAb IgG XP® Isotype Control (Sepharose Bead Conjugate) #3423. The western blot was probed using Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733 and Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 as a secondary antibody.利用Tri-Methyl-Histone H3(Lys27)(C36B11)Rabbit mAb (Sepharose Bead Conjugate) 和Rabbit (DA1E) mAb IgG XP® Isotype Control (Sepharose Bead Conjugate) #3423对HeLa细胞提取物进行免疫沉淀,并使用Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733 和 Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127作为二抗进行Western Blot 检测。.

Background

The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (1). Histone methylation is a major determinant for the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development (2,3). Arginine methylation of histones H3 (Arg2, 17, 26) and H4 (Arg3) promotes transcriptional activation and is mediated by a family of protein arginine methyltransferases (PRMTs), including the co-activators PRMT1 and CARM1 (PRMT4) (4). In contrast, a more diverse set of histone lysine methyltransferases has been identified, all but one of which contain a conserved catalytic SET domain originally identified in the Drosophila Su(var)3-9, Enhancer of zeste, and Trithorax proteins. Lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing (4). Methylation of these lysine residues coordinates the recruitment of chromatin modifying enzymes containing methyl-lysine binding modules such as chromodomains (HP1, PRC1), PHD fingers (BPTF, ING2), tudor domains (53BP1), and WD-40 domains (WDR5) (5-8). The discovery of histone demethylases such as PADI4, LSD1, JMJD1, JMJD2, and JHDM1 has shown that methylation is a reversible epigenetic marker (9).

核小体是构成染色质的基本结构单位,由四种核心组蛋白(H2A、H2B、H3和H4)组成。最初认为组蛋白的功能是作为DNA包装的静态骨架,但现在研究表明,组蛋白是一个动态的蛋白,参与了多种类型的翻译后修饰,包括乙酰化,磷酸化,甲基化以及泛素化(1)。组蛋白甲基化是基因组活动和非活动区域形成的一个重要决定因素,同时对发育过程中基因组的正确编程也是至关重要的(2,3)。位于组蛋白H3(Arg2,17,26)和H4 (Arg3)的精氨酸甲基化能够促进转录激活,该甲基化作用是由蛋白精氨酸甲基转移酶 (PRMTs)家族催化的,其中包括共激活因子 PRMT1 和 CARM1 (PRMT4)(4)。与之不同的是,组蛋白赖氨酸甲基转移酶家族更多样化,除了其中一个,其它所有成员都包含一个保守的SET催化结构域,该SET域(Su(var)3-9, Enhancer of zeste以及Trithorax蛋白)最初是在果蝇中得到确认的。赖氨酸甲基化主要发生于组蛋白 H3 (Lys4,9、 27、 36、 79) 和 H4 (Lys20), 与转录激活和沉默都直接相关 (4)。这些赖氨酸残基的甲基化能够协调染色质修饰酶的募集,这些染色质修饰酶都含有甲基化赖氨酸结合模块,比如染色质结构域(HP1, PRC1), PHD指纹 (BPTF, ING2), tudor 结构域 (53BP1)以及 WD-40 结构域(WDR5) (5-8)。经过研究,人们发现了如PADI4,LSD1,JMJD1,JMJD2, 以及 JHDM1等组蛋白去甲基化酶,这些都表明甲基化修饰是一个可逆的表观遗传学标志。

  1. Peterson, C.L. and Laniel, M.A. (2004) Curr Biol 14, R546-51.
  2. Kubicek, S. et al. (2006) Ernst Schering Res Found Workshop , 1-27.
  3. Lin, W. and Dent, S.Y. (2006) Curr Opin Genet Dev 16, 137-42.
  4. Lee, D.Y. et al. (2005) Endocr Rev 26, 147-70.
  5. Daniel, J.A. et al. (2005) Cell Cycle 4, 919-26.
  6. Shi, X. et al. (2006) Nature 442, 96-9.
  7. Wysocka, J. et al. (2006) Nature 442, 86-90.
  8. Wysocka, J. et al. (2005) Cell 121, 859-72.
  9. Trojer, P. and Reinberg, D. (2006) Cell 125, 213-7.

Application References

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Protocols


For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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