Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) Antibody #4228

p55PIK   Phosphatidylinositol 3-kinase regulatory subunit gamma   PI3-kinase p85-subunit gamma   PIK3R3   PtdIns-3-kinase p85-gamma  

No. Size Price
4228L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
4228S 100 µl ( 10 western blots ) ¥3,780.00 现货查询 购买询价
4228T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
4228 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Mouse, Endogenous 60 and 85 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Homology

Species predicted to react based on 100% sequence homology: Human, Rat, Monkey, Bovine,

Specificity / Sensitivity

Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) Antibody detects endogenous levels of p85/p55 only when phosphorylated at Tyr458/Tyr199.

Phospho-PI3 Kinase p85(Tyr458)/p55 (Tyr199)抗体可以检测内源性在Tyr458/Tyr199发生磷酸化的p85/p55含量。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr458 of mouse p85. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是采用小鼠p85的Tyr458周围残基相对应的合成磷酸肽段免疫动物生产的。该抗体是通过蛋白A和肽亲和色谱法纯化的。

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3-Src cells, untreated or treated with lambda phosphatase and from C2C12 cells, untreated or treated with H2O2, using Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) Antibody.

Western blot分析NIH/3T3-Src细胞提取物,分为应用C2C12细胞γ-磷酸酶处理与非处理组,以及H2O2处理与非处理组,所使用的抗体是Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) Antibody。

Background

Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP) and phosphatidylinositol-4,5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival (1). PTEN reverses this process, and the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN (2). PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit. Various isoforms of the catalytic subunit (p110α, p110β, p110γ, and p110δ) have been isolated, and the regulatory subunits that associate with p110α, p110β, and p110δ are p85α and p85β (3). In contrast, p110γ associates with a p101 regulatory subunit that is unrelated to p85. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins (4).Protein extracts from 3T3-Src cells were profiled by PhosphoScan® to identify phosphotyrosine peptides. Tyr458 of PI3K p85 and Tyr199 of PI3K p55 were among 180 phosphopeptides and 185 phosphotyrosine sites identified (5).

PI3K激酶通过磷酸化磷脂酰肌醇(PI), 4-磷酸磷脂酰肌醇 (PIP)和4,5-二磷酸磷脂酰肌醇 (PIP2)催化3,4,5-三磷酸磷脂酰肌醇的产生。生长因子和激素会引发该磷酸化进程,随后协助细胞生长,细胞周期进入,细胞迁移和细胞存活(1)。PTEN会逆转该过程,研究表明PI3K信号通路在PTEN功能缺失的人类癌症中是持续性激活的(2)。PI3Ks由一个催化亚基(p110)和一个调控亚基构成。已经分离得到了多个催化亚基(p110α, p110β, p110γ, 和p110δ),与p110α, p110β, 和 p110δ结合的调控亚基是p85α 和 p85β (3)。相对的,与p101调控亚基结合的p110γ与p85无关。此外,异源三聚体G蛋白的βγ亚基可以激活p110γ(4)。3T3-Src 细胞蛋白提取物采用PhosphoScan®鉴定酪氨酸磷酸化肽段,PI3K p85的 Tyr458和PI3K p55 的 Tyr199存在于从大约180个磷酸化肽段和185个酪氨酸磷酸化肽段位点。

  1. Cantley, L.C. (2002) Science 296, 1655-7.
  2. Simpson, L. and Parsons, R. (2001) Exp Cell Res 264, 29-41.
  3. Neri, L.M. et al. (2002) Biochim Biophys Acta 1584, 73-80.
  4. Stoyanov, B. et al. (1995) Science 269, 690-3.
  5. Rush, J. et al. (2005) Nat. Biotechnol. 23, 94-101.

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