Cell Signaling Technology

Product Pathways - Neuroscience

Clusterin (D4B6P) Rabbit mAb #42143

sc-166831   sc-6420  

No. Size Price
42143S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
42143 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 32-42, 65, 75 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Clusterin (D4B6P) Rabbit mAb recognizes endogenous levels of total clusterin precursor protein, mature clusterin α chain, and clusterin heterodimer protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His392 of human clusterin protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from concentrated, serum-free culture medium of MCF7 and U-118 MG cells using Clusterin (D4B6P) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF7 and Huh7 cells using Clusterin (D4B6P) Rabbit mAb.

IP

IP

Immunoprecipitation of clusterin from MCF7 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Clusterin (D4B6P) Rabbit mAb. Western blot analysis was performed using Clusterin (D4B6P) Rabbit mAb and Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678.

Background

Clusterin (CLU, apolipoprotein J) is a multifunctional glycoprotein that is expressed ubiquitously in most tissues. Clusterin functions as a secreted chaperone protein that interacts with and stabilizes stress-induced proteins to prevent their precipitation (1,2). Research studies show that clusterin plays a protective role in Alzheimer’s disease by sequestering amyloid β(1-40) peptides to form long-lived, stable complexes, which prevents amyloid fibril formation (3-5).

In addition to the secreted protein, several intracellular isoforms are localized to the nucleus, mitochondria, cytoplasm, and ER. The subcellular distribution of these multiple isoforms leads to the diversity of clusterin functions. Additional studies report that clusterin is involved in membrane recycling, cell adhesion, cell proliferation, apoptosis, and tumor survival (6-9). The clusterin precursor is post-translationally cleaved into the mature clusterin α and clusterin β forms. Clusterin α and β chains create a heterodimer through formation of disulfide bonds (10).

  1. Poon, S. et al. (2000) Biochemistry 39, 15953-60.
  2. Poon, S. et al. (2002) FEBS Lett 513, 259-66.
  3. Yerbury, J.J. et al. (2007) FASEB J 21, 2312-22.
  4. Narayan, P. et al. (2012) Nat Struct Mol Biol 19, 79-83.
  5. Desikan, R.S. et al. (2014) JAMA Neurol 71, 180-7.
  6. Rosenberg, M.E. and Silkensen, J. (1995) Int J Biochem Cell Biol 27, 633-45.
  7. Wilson, M.R. and Easterbrook-Smith, S.B. (2000) Trends Biochem Sci 25, 95-8.
  8. Trougakos, I.P. and Gonos, E.S. (2002) Int J Biochem Cell Biol 34, 1430-48.
  9. Shannan, B. et al. (2006) Cell Death Differ 13, 12-9.
  10. de Silva, H.V. et al. (1990) Biochemistry 29, 5380-9.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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