Cell Signaling Technology

Product Pathways - Metabolism

ABCG2 (D5V2K) XP® Rabbit mAb #42078

sc-58224  

No. Size Price
42078S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
42078 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 65-80 Rabbit IgG
IP 1:50
IHC-P 1:350
F 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry,

Specificity / Sensitivity

ABCG2 (D5V2K) XP® Rabbit mAb recognizes endogenous levels of total ABCG2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp175 of human ABCG2 protein.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human placenta using ABCG2 (D5V2K) XP® Rabbit mAb in the presence of control peptide (left) and antigen-specific peptide (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded normal human uterus using ABCG2 (D5V2K) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded A549 (left) and A-204 (right) cell pellets using ABCG2 (D5V2K) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using ABCG2 (D5V2K) XP® Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HEK293 (blue) and A549 (green) cells using ABCG2 (D5V2K) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from RPMI 8226 and A549 cells and human placenta using ABCG2 (D5V2K) XP® Rabbit mAb.

IP

IP

Immunoprecipitation of ABCG2 from A549 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is ABCG2 (D5V2K) XP® Rabbit mAb. Western blot analysis was performed using ABCG2 (D5V2K) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse kidney using ABCG2 (D5V2K) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse liver using ABCG2 (D5V2K) XP® Rabbit mAb.

Background

ABCG2 (BCRP1/ABCP/MXR) is a member of the ATP-binding cassette transporter family that functions as ATP-dependent transporters for a wide variety of chemical compounds and are associated with drug-resistance in cancer cells (1-6). ABCG2 is a heavily glycosylated transmembrane protein with six transmembrane spanning regions consistent with it functioning as a half-transporter. The ABC family can exist as either full-length transporters or as half-transporters that form functional transporters through homo- or heterodimerization. High expression of ABCG2 was found in placenta as well as cell lines selected for resistance to a number of chemotherapeutic drugs, including mitoxantrone, doxorubicin, topotecan and flavopiridol. In rodents, the highest expression of ABCG2 was found in kidney (8). ABCG2 expression has also been observed in stem cell populations, particularly in hematopoietic and neuronal stem cells and is downregulated with differentiation (9-12).

  1. Doyle, L.A. and Ross, D.D. (2003) Oncogene 22, 7340-58.
  2. Allen, J.D. et al. (1999) Cancer Res 59, 4237-41.
  3. Doyle, L.A. et al. (1998) Proc Natl Acad Sci U S A 95, 15665-70.
  4. Allikmets, R. et al. (1998) Cancer Res 58, 5337-9.
  5. Miyake, K. et al. (1999) Cancer Res 59, 8-13.
  6. Robey, R.W. et al. (2001) Clin Cancer Res 7, 145-52.
  7. Zhou, S. et al. (2001) Nat Med 7, 1028-34.
  8. Honscha, W. et al. (2000) Hepatology 31, 1296-304.
  9. Scharenberg, C.W. et al. (2002) Blood 99, 507-12.
  10. Islam, M.O. et al. (2005) Neurosci Res 52, 75-82.
  11. Bunting, K.D. (2002) Stem Cells 20, 11-20.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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