Cell Signaling Technology

Product Pathways - Neuroscience

Phospho-NMDAR2A (Tyr1246) Antibody #4206


No. Size Price
4206S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
4206 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Rat, Endogenous 180 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,


Species predicted to react based on 100% sequence homology: Human, Mouse,

Specificity / Sensitivity

Phospho-NMDAR2A (Tyr1246) Antibody detects endogenous levels of NMDAR2A only when phosphorylated at Tyr1246. The antibody may also detect NMDAR2B when phosphorylated at the conserved Tyr1252.

Phospho-NMDAR2A (Tyr1246) Antibody 兔多抗识别内源性的Tyr1246磷酸化的NMDAR2A蛋白。它也可以识别Tyr1252磷酸化的NMDAR2B蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1246 of human NMDAR2A. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from rat brain, either sham-operated or 15 min ischemia, using Phospho-NMDAR2A (Tyr1246) Antibody (upper) or NMDAR2A Antibody (lower).

Western blot分析大鼠大脑提取物,假手术组或15分钟缺血,使用的抗体是Phospho-NMDAR2A (Tyr1246) Antibody 兔多抗(上图)或NMDAR2A Antibody 兔多抗(下图)。


N-methyl-D-aspartate receptor (NMDAR) forms a heterodimer of at least one NR1 and one NR2A-D subunit. Multiple receptor isoforms with distinct brain distributions and functional properties arise by selective splicing of the NR1 transcripts and differential expression of the NR2 subunits. NR1 subunits bind the co-agonist glycine and NR2 subunits bind the neurotransmitter glutamate. Activation of the NMDA receptor or opening of the ion channel allows flow of Na+ and Ca2+ ions into the cell, and K+ out of the cell (1). Each subunit has a cytoplasmic domain that can be directly modified by the protein kinase/phosphatase (2). PKC can phosphorylate the NR1 subunit (NMDAR1) of the receptor at Ser890/Ser896, and PKA can phosphorylate NR1 at Ser897 (3). The phosphorylation of NR1 by PKC decreases its affinity for calmodulin, thus preventing the inhibitory effect of calmodulin on NMDAR (4). The phosphorylation of NR1 by PKA probably counteracts the inhibitory effect of calcineurin on the receptor (5). NMDAR mediates long-term potentiation and slow postsynaptic excitation, which play central roles in learning, neurodevelopment, and neuroplasticity (6).EphrinB2 binding to the receptor EphB leads to the activation of Src family tyrosine kinases, which phosphorylate NMDAR2B at Tyr1252, Tyr1336 and Tyr1472. In turn, phosphorylated NMDAR2B enhances the ability of the functional NMDA receptor to regulate Ca2+ influx in response to glutamate (7). The phosphorylation site of NMDAR2A at Tyr1246 is the conserved site of NMDAR2B at Tyr1252 and was independently identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's MS/MS platform for phosphorylation site discovery. Phosphorylation of NMDAR2A at Tyr1246 was observed in extracts isolated from ischemic rat brain. For additional information please visit PhosphoSitePlus®, CST's modification site knowledgebase, at www.phosphosite.org.

N-甲基-D-天冬氨酸(NMDA)受体形成至少有一个NR1和一个NR2A-D亚基的异二聚体。其各种在脑内分布和功能特性不同的亚型是由NR1的选择性剪切和NR2的差异表达所形成。NR1亚基结合的共刺激激动剂甘氨酸,NR2结合的神经递质谷氨酸。NMDA受体激活或离子通道的开放,允许Na+和Ca2+离子进入细胞和K+流出细胞(1)。每个亚基有一个细胞质域,可直接通过蛋白激酶/磷酸酶进行修饰调控(2)。蛋白激酶C可以在Ser890/Ser896磷酸化NR1亚基(NMDAR1),PKA可以磷酸化NR1的Ser897(3)。NR1的PKC磷酸化降低其对钙调蛋白的亲和力,从而防止钙调蛋白对NMDA受体的抑制作用(4)。PKA磷酸化NR1可能可抵消钙调蛋白对受体的抑制作用(5)。NMDA受体介导长时程增强和慢速突触后兴奋,从而在学习,神经发育和神经可塑性中发挥中心作用(6)。EphrinB2结合EphB受体,导致Src家族络氨酸激酶的激活,从而磷酸化NMDAR2B的Tyr1252, Tyr1336和Tyr1472位点。磷酸化的NMDAR2B增强NMDA受体在接受谷氨酸能刺激时调节Ca2+内流的能力(7)。NMDAR2A的Tyr1246磷酸化位点与Tyr1252保守,由CST公司使用PhosphoScan®和CST的磷酸化位点质谱平台分别发现。NMDAR2A在Tyr1246上的磷酸化在缺血大鼠脑组织分离提取物中可见。更多信息,请参见PhosphoSitePlus®,CST修饰位点知识库,www.phosphosite.org。

  1. Liu, X.B. et al. (2004) J Neurosci 24, 8885-95.
  2. Westphal, R.S. et al. (1999) Science 285, 93-6.
  3. Tingley, W.G. et al. (1997) J Biol Chem 272, 5157-66.
  4. Hisatsune, C. et al. (1997) J Biol Chem 272, 20805-10.
  5. Raman, I.M. et al. (1996) Neuron 16, 415-21.
  6. Makhinson, M. et al. (1999) J Neurosci 19, 2500-10.
  7. Takasu, M.A. et al. (2002) Science 295, 491-495.

Application References

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