Cell Signaling Technology

Product Pathways - Apoptosis

Cleaved Caspase-1 (Asp297) (D57A2) Rabbit mAb #4199

sc-398715   sc-514   sc-515   sc-56036  

No. Size Price
4199S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4199T 20 µl ( 2 western blots ) ¥1,300.00 现货查询 购买询价
4199 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 20, 22 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Homology

Species predicted to react based on 100% sequence homology: Monkey,

Specificity / Sensitivity

Cleaved Caspase-1 (Asp297) (D57A2) Rabbit mAb detects endogenous levels of the p20 subunit of human caspase-1 only upon cleavage at Asp297.

Cleaved Caspase-1 (Asp297) (D57A2) Rabbit mAb 兔单抗只能够检测内源水平的Asp297位点剪切的人源caspase-1的 p20 亚单元。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues adjacent to Asp297 of human caspase-1.

该单克隆抗体是采用合成人caspase-1 链接Asp297残基相对应的肽段来免疫动物而获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7 cells, untransfected (-) or transfected with construct overexpressing human caspase-1 (+), using Cleaved Caspase-1 (Asp297) (D57A2) Rabbit mAb (upper) or Caspase-1 (D7F10) Rabbit mAb #3866 (lower).

Western blot分析COS-7细胞的细胞提取液,未转染(-)或转染了过表达人caspase-1的质粒 (+),所用抗体是Cleaved Caspase-1 (Asp297) (D57A2) Rabbit mAb 兔单抗(上图) 或者 Caspase-1 (D7F10) Rabbit mAb 兔单抗#3866 (下图)

Western Blotting

Western Blotting

Western blot analysis of extracts from the media of THP-1 cells, differentiated with TPA #9905 (80 nM, overnight) followed by treatment with LPS (1 mu-g/ml, 8 hours), using Cleaved Caspase-1 (Asp297) (D57A2) Rabbit mAb.

Western blot分析 THP-1细胞的提取液,细胞经TPA #9905 (80 nM, 过夜)分化,随后用LPS (1 mu-g/ml, 8 hours)处理。用Cleaved Caspase-1 (Asp297) (D57A2) Rabbit mAb研究

Background

Caspase-1, or interleukin-1ß converting enzyme (ICE/ICEα), is a class I cysteine protease, which also includes caspases -4, -5, -11, and -12. Caspase-1 cleaves inflammatory cytokines such as pro-IL-1ß and interferon-γ inducing factor (IL-18) into their mature forms (1,2). Like other caspases, caspase-1 is proteolytically activated from a proenzyme to produce a tetramer of its two active subunits, p20 and p10. Caspase-1 has a large amino-terminal pro-domain that contains a caspase recruitment domain (CARD). Overexpression of caspase-1 can induce apoptosis (3). Mice deficient in caspase-1, however, have no overt defects in apoptosis but do have defects in the maturation of pro-IL-1β and are resistant to endotoxic shock (4,5). At least six caspase-1 isoforms have been identified, including caspase-1 α, β, γ, δ, ε and ζ (6). Most caspase-1 isoforms (α, β, γ and δ) produce products between 30-48 kDa and induce apoptosis upon over-expression. Caspase-1 ε typically contains only the p10 subunit, does not induce apoptosis and may act as a dominant negative. The widely expressed ζ isoform of caspase-1 induces apoptosis and lacks 39 amino-terminal residues found in the α isoform (6). Activation of caspase-1 occurs through an oligomerization molecular platform designated the "inflammasome" that includes caspase-5, Pycard/Asc, and NALP1 (7).

Caspase-1,又称白介素-1ß转化酶(ICE/ICEα),是一种 I 类半胱氨酸蛋白酶,I类半胱氨酸蛋白酶还包括caspases -4、-5、-11 和-12。Caspase-1 能够将炎症细胞因子如pro-IL-1ß 和干扰素-γ诱导因子 (IL-18) 剪切成成熟蛋白 (1,2)。与其它的 caspases 一样, caspase-1通过蛋白水解被激活,酶原产生一个由两个活性亚单元p20和 p10组成的四聚体。Caspase-1具有巨大的N-末端前体域,此区域包含一个caspase 招募区域(CARD)。过表达caspase-1 能诱导凋亡(3)。Caspase-1 缺失的小鼠,在凋亡方面没有严重缺陷,但是却在pro-IL-1β 蛋白的成熟过程中有严重缺陷,而且这个小鼠对内毒素有抗性(4,5)。研究已经证实 Caspase-1 至少有六种形式,包括caspase-1 α、β、γ、δ、ε和ζ (6)。大多数caspase-1 (α,β,γ和 δ) 产生的产物在 30-48 kDa之间,并通过过表达诱导凋亡。Caspase-1ε 通常只包含p10亚单元,是没有诱导凋亡活性的显性失活蛋白。caspase-1中广泛表达的ζ形式诱导凋亡,并且比较α形式,其在N-末端缺少39个氨基酸残基(6)。Caspase-1激活的发生是通过一个特殊的“炎症小体”的多聚分子平台,此中包含 caspase-5、Pycard/Asc和 NALP1 (7)。

  1. Thornberry, N.A. et al. (1992) Nature 356, 768-74.
  2. Martinon, F. and Tschopp, J. (2004) Cell 117, 561-74.
  3. Miura, M. et al. (1993) Cell 75, 653-60.
  4. Kuida, K. et al. (1995) Science 267, 2000-3.
  5. Li, P. et al. (1995) Cell 80, 401-11.
  6. Feng, Q. et al. (2004) Genomics 84, 587-91.
  7. Martinon, F. et al. (2002) Mol Cell 10, 417-26.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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