Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Phospho-Stathmin (Ser38) (D19H10) Rabbit mAb #4191


No. Size Price
4191S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4191T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
4191 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 19, 20 Rabbit IgG
IP 1:200
IHC-P 1:8000
IF-IC 1:12800

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Phospho-Stathmin (Ser38) (D19H10) Rabbit mAb detects endogenous levels of stathmin protein only when phosphorylated at Ser38.

Phospho-Stathmin (Ser38) (D19H10) Rabbit mAb检测仅在Ser38位点磷酸化的内源性stathmin蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser38 of human stathmin protein.


Western Blotting

Western Blotting

Western blot analysis of extracts from HT-29 and U-2 OS cells, untreated or synchronized in mitosis, using Phospho-Stathmin (Ser38) (D19H10) Rabbit mAb. Mitotic synchrony was performed by using either a thymidine block followed by release into 100 ng/mL nocodazole for 24 hours or using 100 ng/mL Docetaxel #9886 for 24 hours.

使用Phospho-Stathmin (Ser38) (D19H10) Rabbit mAb,免疫印迹(Western Blot)分析HT-29和U-2 OS细胞中Phospho-Stathmin蛋白水平。细胞分为untreated或Mitotic synchrony。Mitotic synchrony通过使用thymidine封闭随后100 ng/mL nocodazole处理24小时或使用100 ng/mL Docetaxel #9886 处理24小时。

Western Blotting

Western Blotting

Western blot analysis of extracts from HT-29 cells, untreated or treated with nocodazole alone (100 ng/mL 24 hours) or nocodazole followed by λ and calf intestinal phosphatases, using Phospho-Stathmin (Ser38) (D19H10) Rabbit mAb.

使用Phospho-Stathmin (Ser38) (D19H10) Rabbit mAb,免疫印迹(Western Blot)分析HT-29细胞中Phospho-Stathmin蛋白水平。细胞分为untreated或nocodazole alone (100 ng/mL 24 hours)或nocodazole随后λ和calf intestinal phosphatases处理。



Confocal immunofluorescent analysis of HT-29 cells, untreated (left) or λ phosphatase-treated (right), using Phospho-Stathmin (Ser38) (D19H10) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

使用Phospho-Stathmin (Ser38) (D19H10) Rabbit mAb (绿色)标记,共聚焦免疫荧光分析HT-29细胞,细胞分为untreated (左图)和λ phosphatase-treated (right)。DY-554 phalloidin标记微丝蛋白(红色)。蓝色伪彩= DRAQ5® #4084 (DNA荧光染料)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded HT-29 cell pellets, untreated (left) or nocodazole-treated (right), using Phospho-Stathmin (Ser38) (D19H10) Rabbit mAb.

使用Phospho-Stathmin (Ser38) (D19H10) Rabbit mAb,免疫组化分析HT-29细胞石蜡切片,组织分为control (左图)或nocodazole-treated (右图)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma control (left) or λ phosphatase-treated (right) using Phospho-Stathmin (Ser38) (D19H10) Rabbit mAb.

使用Phospho-Stathmin (Ser38) (D19H10) Rabbit mAb,免疫组化分析人源乳腺癌组织石蜡切片,组织分为control (左图)或λ phosphatase-treated (右图)。


Stathmin is a ubiquitously expressed microtubule destabilizing phosphoprotein that is upregulated in a number of cancers. The amino terminus of the protein contains multiple phosphorylation sites and is involved in the promotion of tubulin filament depolymerization. Phosphorylation at these sites inactivates the protein and stabilizes microtubules. Ser16 phosphorylation by CaM kinases II and IV (1,2) increases during G2/M-phase and is involved in mitotic spindle regulation (3,4). Ser38 is a target for cdc2 kinase (5) and TNF-induced cell death gives rise to reactive oxygen intermediates leading to hyperphosphorylation of stathmin (6). EGF receptor activation of Rac and cdc42 also increases phosphorylation of stathmin on Ser16 and Ser38 (7). Other closely related family members are neuronally expressed and include SCG10, SCLIP, RB3 and its splice variants RB3' and RB3''. Stathmin and SCG10 have been shown to play roles in neuronal-like development in PC12 cells (8).

Stathmin蛋白是一个广泛表达的微管不稳定的磷蛋白(phosphoprotein),该蛋白在许多癌症中被上调。该蛋白的氨基端包含多个磷酸化位点,并且涉及促进tubulin纤维解聚作用。这些位点的磷酸化使该蛋白失活和稳定微管。在G2/M期,通过CaM kinases II and IV(1,2)使Ser16位点磷酸化增加,并涉及有丝分裂纺锤体打的调节(3,4)。Ser38是cdc2激酶的靶蛋白 (5),并且TNF诱导的细胞死亡引起活性氧中间产物,这导致stathmin蛋白的过度磷酸化(6)。Rac和cdc42蛋白的EGF受体激活也增加stathmin蛋白在Ser16和Ser38位点的磷酸化(7)。其它紧密相关的家族成员是神经元性表达,并且包括SCG10、SCLIP、RB3和它的剪切体RB3'、RB3''。Stathmin和SCG10蛋白已经证明在PC12细胞神经样发育中起着一定作用(8)。

  1. Marklund, U. et al. (1994) Eur J Biochem 225, 53-60.
  2. le Gouvello, S. et al. (1998) J Immunol 161, 1113-22.
  3. Mistry, S.J. and Atweh, G.F. (2001) J Biol Chem 276, 31209-15.
  4. Gavet, O. et al. (1998) J Cell Sci 111 ( Pt 22), 3333-46.
  5. Luo, X.N. et al. (1994) J Biol Chem 269, 10312-8.
  6. Vancompernolle, K. et al. (2000) J Biol Chem 275, 33876-82.
  7. Daub, H. et al. (2001) J Biol Chem 276, 1677-80.
  8. Di Paolo, G. et al. (1996) J Cell Biol 133, 1383-90.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :