Cell Signaling Technology

Product Pathways - Metabolism

Acetyl-CoA Carboxylase 1 Antibody #4190

ACAC   ACACA   ACC   ACC-alpha   ACC1   ACCA   ACCalpha   Acetyl-CoA   Acetyl-CoA Carboxylase 1   Carboxylase   Carboxylase 1  

No. Size Price
4190S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
4190 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 265 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Acetyl-CoA Carboxylase 1 Antibody detects endogenous levels of total acetyl-CoA carboxylase 1 protein. Immunofluorescence data indicate that the antibody is more reactive to rodent than human proteins.

Acetyl-CoA Carboxylase 1 Antibody用于检测内源性acetyl-CoA carboxylase 1总蛋白水平。荧光免疫实验表明,该抗体对鼠类蛋白比对人类蛋白反应更敏感。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human acetyl-CoA carboxylase 1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

多克隆抗体通过用对应于人源acetyl-CoA carboxylase 1蛋白序列的合成肽免疫动物制备而得,抗体经蛋白A和肽段亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Acetyl-CoA Carboxylase 1 Antibody.

Wetern blot检测多种细胞提取物,所用抗体为Acetyl-CoA Carboxylase 1 Antibody



Confocal immunofluorescent analysis of HCC827 (left) and C2C12 cells (right) using Acetyl-CoA Carboxylase 1 Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

激光共聚焦免疫荧光检测HCC827 (左) and C2C12 细胞(右) ,所用抗体为Acetyl-CoA Carboxylase 1 Antibody(绿),蓝色伪彩为DNA荧光染料(对应产品号为DRAQ5 #4084).

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, mock transfected or transfected with either SignalSilence® ACC1 siRNA or SignalSilence® ACC2 siRNA, using Acetyl-CoA Carboxylase 1 Antibody and β-Actin (13E5) Rabbit mAb #4970.

Wetern blot检测hela细胞提取物,细胞空转或用SignalSilence® ACC1 siRNA 或 SignalSilence® ACC2 siRNA转染,所用抗体为Acetyl-CoA Carboxylase 1 Antibody和β-Actin (13E5) Rabbit mAb兔单抗 #4970.


Acetyl-CoA carboxylase (ACC) catalyzes the pivotal step of the fatty acid synthesis pathway. The 265 kDa ACCα (ACC1) is the predominant isoform found in liver, adipocytes, and mammary gland, while the 280 kDa ACCβ (ACC2) is the major isoform in skeletal muscle and heart (1). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (2). ACC is a potential target of anti-obesity drugs (3,4).

乙酰辅酶A合成酶(ACC)用于催化脂肪酸合成途径中的关键步骤。分子量为265 kDa的ACCα,是肝脏,脂肪细胞和乳腺中ACC的主要形式,而在骨胳肌和心脏中,其主要形式为280 kDa的ACCβ(1)。ACC可以在Ser79被AMPK磷酸化,或者在Ser1200被PKA磷酸化,这些位点的磷酸化可以抑制ACC的酶活性(2)。ACC也是减肥药物的一个潜在靶点(3,4)。

  1. Castle, J.C. et al. (2009) PLoS One 4, e4369.
  2. Kreuz, S. et al. (2009) Diabetes Metab Res Rev 25, 577-86.
  3. Ha, J. et al. (1994) J Biol Chem 269, 22162-8.
  4. Abu-Elheiga, L. et al. (2001) Science 291, 2613-6.
  5. Levert, K.L. et al. (2002) J Biol Chem 277, 16347-50.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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