Cell Signaling Technology

Product Pathways - Development

Phospho-β-Catenin (Ser675) (D2F1) XP® Rabbit mAb #4176

No. Size Price
4176S 100 µl ( 10 western blots ) ¥4,180.00 现货查询 购买询价 防伪查询
4176T 20 µl ( 2 western blots ) ¥1,600.00 现货查询 购买询价 防伪查询
4176 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 92 Rabbit IgG
IP 1:200
IF-F 1:100
IF-IC 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-F=Immunofluorescence (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry),


Species predicted to react based on 100% sequence homology: Mouse, Rat, Chicken, Xenopus, Zebrafish,

Specificity / Sensitivity

Phospho-β-Catenin (Ser675) (D2F1) XP® Rabbit mAb detects endogenous levels of β-catenin only when phosphorylated at Ser675.

磷酸化-β-Catenin (Ser675) (D2F1) XP®的兔单抗仅识别Ser675磷酸化的内源性β-Catenin蛋白.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser675 of human β-catenin.




Confocal immunofluorescent analysis of HeLa cells, untreated (left), λ phosphatase-treated (middle), or untreated NCI-H28 cells (β-catenin null; right) using Phospho-β-Catenin (Ser675) (D2F1) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

对未处理(左)或λ-磷酸酶处理(中)的HeLa细胞,或未经处理的NCI-H28细胞(β-catenin 缺失;右)使用Phospho-β-Catenin (Ser675) (D2F1) XP® Rabbit mAb (绿色)进行激光共聚焦荧光分析。肌动蛋白丝使用DY-554phalloidin(红色)标记.蓝色伪彩=DRAQ5® #4084 (DNA荧光染料).

Western Blotting

Western Blotting

Western blot analysis of extracts from MKN-45 and SK-N-MC cells, untreated or treated with λ phosphatase for 1 hour or forskolin (FSK) for 30 minutes, using Phospho-β-Catenin (Ser675) (D2F1) XP® Rabbit mAb (upper) or β-Catenin (6B3) Rabbit mAb #9582 (lower).

MKN-45 and SK-N-MC 细胞,未处理或经λ磷酸酶处理1小时或用福斯科林(FSK)处理30分钟,获得细胞提取物,使用Phospho-β-Catenin (Ser675) (D2F1) XP® Rabbit mAb(上) 或β-Catenin (6B3) Rabbit mAb #9582 (下)进行western blot分析。



Confocal immunofluorescent analysis of rat colon using Phospho-β-Catenin (Ser675) (D2F1) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 Phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

使用Phospho-β-Catenin (Ser675) (D2F1) XP® Rabbit mAb (绿色)对大鼠结肠组织进行激光共聚焦荧光分析. 肌动蛋白丝使用DY-554 Phalloidin(红色)标记。蓝色伪彩=DRAQ5® #4084 (DNA荧光染料).


β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).

β-catenin是Wnt信号通路下游的重要效应分子(1)。在脊椎动物体内它涉及到两个重要的生物过程:早期胚胎发育(2)和肿瘤发生(3)。CK1可以磷酸化β-catenin 45位丝氨酸。该磷酸化将导致β-catenin能够随后被GSK-3磷酸化(4-6)。GSK-3β通过磷酸化β-catenin Ser33, Ser37, 和 Thr41,并促使其进一步被降解(7)。这些位点发生突变会提高β-catenin蛋白稳定性,并且已在多种癌细胞株中发现这些突变(8)。

PKA was shown to phosphorylate β-catenin at Ser675. Phosphorylation at Ser675 induces β-catenin accumulation in the nucleus and increases its transcriptional activity (9,10).

PKA磷酸化β-Catenin的675位丝氨酸,此位点的磷酸化诱导β-catenin在核内的累积,增加它的转录活性(9,10) .

  1. Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305.
  2. Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88.
  3. Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21.
  4. Amit, S. et al. (2002) Genes Dev 16, 1066-76.
  5. Liu, C. et al. (2002) Cell 108, 837-47.
  6. Yanagawa, S. et al. (2002) EMBO J 21, 1733-42.
  7. Yost, C. et al. (1996) Genes Dev 10, 1443-54.
  8. Morin, P.J. et al. (1997) Science 275, 1787-90.
  9. Taurin, S. et al. (2006) J Biol Chem 281, 9971-6.
  10. Hino, S. et al. (2005) Mol Cell Biol 25, 9063-72.

Application References

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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