Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174

phorbol ester   PKC   PMA   protein kinase C   sc-3576  

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Description

TPA (12-O-tetradecanoylphorbol-13-acetate)/ PMA (phorbol-12-myristate-13-acetate) is soluble in DMSO and ethanol. DMSO solutions can be stored at -20C, in the absence of light, for at least six months. A 200 nM TPA treatment is standard to induce signaling.

TPA(12-O-tetradecanoylphorbol-13-acetate)/ PMA(phorbol-12-myristate-13-acetate)溶于DMSO和乙醇。DMSO溶液可在-20℃避光保存至少六个月。200nM TPA处理是诱导信号的标准处理。

Western Blotting

Western Blotting

Western blot analysis of extracts from serum-starved 293 cells, untreated (lane 1), stimulated with TPA treatment (lanes 2 & 3), and treated with CIP and λ phosphatase (lane 3), using Phospho-PKC (pan) (βII Ser660) Antibody #9371 or PKCα Antibody #2056.

对血清饥饿的293细胞,未处理(列1),0.2uM TPA处理20分钟(列2,3),CIP/λ磷酸酶处理(列3),使用Phospho-PKC (pan) (βII Ser660)抗体#9371或PKCα抗体#2056进行Western blot分析。

Western Blotting

Western Blotting

Western blot analysis of extracts from serum-starved 293 cells, untreated (lane 1), stimulated with TPA treatment (lanes 2 & 3), and treated with CIP and λ phosphatase (lane 3), using Phospho-PKD/PKCμ (Ser916) Antibody #2051 or PKD/PKCμ Antibody #2052.

对血清饥饿的293细胞,未处理(列1),0.2uM TPA处理20分钟(列2,3),CIP/λ磷酸酶处理(列3),使用Phospho-PKD/PKCμ (Ser916) 抗体#2051或PKD/PKCμ抗体#2052进行Western blot分析。

Directions for Use

TPA is supplied as a 1 mg powder. Store at -20C. Before use, dissolve powder in 1 ml DMSO to yield a 1.6 mM stock solution. For working concentration of 200 μM, add 125 μl stock solution to 875 μl DMSO. Treat cells with 200 nM TPA for 20-30 minutes.

TPA以1mg粉末提供。储存在-20℃。使用前,将粉末溶解于1ml DMSO中产生1.6mM原液。对于工作浓度为200uM,将125ul原液加入至875ul DMSO中。200nM TPA处理细胞20-30分钟。

Background

TPA (12-O-tetradecanoylphorbol-13-acetate) / PMA (phorbol-12-myristate-13-acetate) is the most commonly used phorbol ester. It binds and activates protein kinase C, causing a wide range of effects in cells and tissues (1,2). TPA has been demonstrated to be a potent tumor promoter in mouse skin (3). Conversely, it has been shown to be an effective cancer therapeutic agent in myelocytic leukemia patients, and has been indicated as a potential colorectal cancer therapeutic (4-5). TPA has also been shown to increase white blood cell and neutrophil counts in solid tumor cancer patients (6).

TPA (12-O-tetradecanoylphorbol-13-acetate) / PMA (phorbol-12-myristate-13-acetate)是最常用的佛波酯。它结合并激活蛋白激酶C,对很多细胞和组织产生影响(1,2)。TPA已被证明在在小鼠皮肤上是一个强有力的促癌物(3)。相反,它已被证明是粒细胞白血病的一种有效的治疗药物,在大肠癌治疗中也有潜在疗效(4-5)。TPA也被证明能在实质性癌症患者中增加白细胞和中性粒细胞(6)。

  1. Rebois, R.V. and Patel, J. (1985) J Biol Chem 260, 8026-31.
  2. Blumberg, P.M. (1980) Crit Rev Toxicol 8, 153-97.
  3. Fürstenberger, G. et al. (1981) Proc Natl Acad Sci U S A 78, 7722-6.
  4. Han, Z.T. et al. (1998) Proc Natl Acad Sci U S A 95, 5357-61.
  5. Lee, H.W. et al. (2002) J Biol Chem 277, 32624-31.
  6. Han, Z.T. et al. (1998) Proc Natl Acad Sci U S A 95, 5362-5.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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