Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-MKK7 (Ser271/Thr275) Antibody #4171

JNKK-2   mkk47  

No. Size Price
4171S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4171 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 48 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Mouse, Rat,

Specificity / Sensitivity

Phospho-MKK7 (Ser171/Thr275) Antibody detects endogenous levels of MKK7 phosphorylated at serine 271 and threonine 275. This antibody does not cross-react with phosphorylated SEK1/MKK4 or other phosphorylated MAP kinase kinases.

Phospho-MKK7 (Ser171/Thr275) Antibody兔多抗能检测内源性Ser271位点和Thr275位点磷酸化的MKK7蛋白水平。该抗体不能与磷酸化的SEK1/MKK4、其他磷酸化的MAPKK发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser271/Thr275 of human MKK7. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis and extracts from THP1, U-937 and Ramos cells, untreated or sorbitol-treated (400mM, 30 min), using Phospho-MKK7 (Ser271/Thr275) Antibody (upper) or MKK7 Antibody #4172 (lower).western blot方法检测细胞提取物:未处理和山梨醇处理(400mM, 30 min)的THP1, U-937、Ramos细胞。使用的抗体是Phospho-MKK7 (Ser271/Thr275) Antibody (上图) 和 MKK7 Antibody #4172 (下图)。


MKK7 is a MAP kinase kinase that serves as a specific activator of JNK/SAPK pathway (1,2). MKK7 is activated strongly by TNFα, as well as other environmental stresses, whereas SEK1/MKK4, which activates both p38 and JNK/SAPK pathways, is not activated by TNFα (2). Sequence alignment of the activation loop of the MAP kinase kinase family members indicates that Ser271 and Thr275 are potential phosphorylation sites that are crucial for the kinase acivity.

MKK7是一种MAPKK,它是JNK/SAPK通路的特异性活化因子(1,2)。MKK7能被TNFα以及其他环境压力显著激活,然而能激活 p38、JNK/SAPK通路的SEK1/MKK4却不能被TNFα所激活(2)。对MAPKK家族成员活化环的序列比对表明,MKK7蛋白的Ser271和Thr275位点是潜在的磷酸化位点,这两个位点对激酶的活性十分重要。

  1. Tournier, C. et al. (1999) Mol. Cell. Biol. 19, 1569-1581.
  2. Moriguchi, T. et al. (1997) EMBO J. 16, 7045-7053.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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