Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

TFIIB (2F6A3H4) Mouse mAb #4169

GTF2B   sc-225   sc-274   TF2B   TFII   transcription factor   transcription initiation  

No. Size Price
4169S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
4169 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 32 Mouse IgG2b
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

TFIIB (2F6A3H4) Mouse mAb detects endogenous levels of total TFIIB protein.

TFIIB (2F6A3H4) Mouse mAb鼠单抗能够检测内源性TFIIB总蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human TFIIB protein.

通过重组人源TFIIB蛋白去免疫动物从而制备出此单克隆抗体。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using TFIIB (2F6A3H4) Mouse mAb.

使用TFIIB (2F6A3H4) Mouse mAb鼠单抗,免疫印迹(Western blot)分析不同细胞系中TFIIB的蛋白水平。

Background

TFIIB (also known as GTF2B, TF2B and general transcription factor IIB) is a ubiquitously expressed transcription factor that plays a central role in the assembly of the transcription pre-initiation complex through direct recruitment of RNA polymerase II (1,2). TFIIB functions as a bridge between promoter-bound TFIID and RNA polymerase II. In addition to interacting with promoter-bound TFIID and TFIIA, TFIIB makes extensive contacts with the core promoter via two independent DNA-binding modules. TFIIB may also be a target of transcriptional activator proteins that act to stimulate pre-initiation complex assembly.

TFIIB (也称为GTF2B、TF2B和general transcription factor IIB)是一个广泛表达的转录因子,它通过直接招募RNA polymerase II在转录起始前复合物的装配中起着关键作用(1,2)。TFIIB功能作为束缚在启动子上的TFIID和RNA polymerase II之间的一个桥梁作用。除了与束缚在启动子TFIID和TFIIA相互作用之外,TFIIB蛋白通过两个独立的DNA结合模板使得与中心启动子有广泛接触。TFIIB蛋白也可能是一个转录激活蛋白的靶蛋白,该蛋白可以刺激起始前复合物的装配。

  1. Deng, W. and Roberts, S.G. (2007) Chromosoma 116, 417-29.
  2. Thomas, M.C. and Chiang, C.M. Crit Rev Biochem Mol Biol 41, 105-78.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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