Cell Signaling Technology

Product Pathways - Neuroscience

Phospho-TrkA (Tyr785)/TrkB (Tyr816) (C67C8) Rabbit mAb #4168

BDNF   NGF   RTK  

No. Size Price
4168S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4168T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
4168 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Rat, Endogenous 140 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Human, Mouse,

Specificity / Sensitivity

Phospho-TrkA (Tyr785)/TrkB (Tyr816) (C67C8) Rabbit mAb detects endogenous levels of TrkA and TrkB when phosphorylated at Tyr785 of TrkA or Tyr816 of TrkB. This antibody may cross-react with other tyrosine-phosphorylated proteins.

Phospho-TrkA (Tyr785)/TrkB (Tyr816) (C67C8)Rabbit mAb兔单抗可识别内源性的Tyr785/Tyr816磷酸化的TrkA/TrkB。此抗体与其它酪氨酸磷酸化蛋白没有交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr785 of human TrkA protein.

该单克隆抗体由合成的人类TrkA蛋白Tyr785位点附近磷酸化肽段免疫动物而制成。

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells transfected with TrkA, untreated or treated with NGF, using Phospho-TrkA (Tyr785)/TrkB (Tyr816) (C67C8) Rabbit mAb (upper) and TrkA (14G6) Rabbit mAb #2508 (lower).

Western blot分析转染了TrkB的NIH/3T3细胞,未处理或使用NGF处理,使用的抗体是Phospho-TrkA (Tyr785)/TrkB (Tyr816) (C67C8)Rabbit mAb兔单抗(上图)和TrkA (14G6)Rabbit mAb 兔单抗#2508(下图)进行。

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells transfected with TrkB, untreated or treated with hBDNF #3897, using Phospho-TrkA (Tyr785)/TrkB (Tyr816) (C67C8) Rabbit mAb (upper) and Trk (pan) (C17F1) Rabbit mAb #4609 (lower).

Western blot分析转染了TrkB的NIH/3T3细胞,未处理或使用hBDNF #3897预处理,然后使用Phospho-TrkA (Tyr785)/TrkB (Tyr816) (C67C8) Rabbit mAb 兔单抗(上图)和Trk (pan) (C17F1) Rabbit mAb 兔单抗#4609(下图)。

Background

The family of Trk receptor tyrosine kinases consists of TrkA, TrkB and TrkC. While the sequence of these family members is highly conserved, they are activated by different neurotrophins: TrkA by NGF, TrkB by BDNF or NT4, and TrkC by NT3. TrkA regulates proliferation and is important for development and maturation of the nervous system (1). Phosphorylation at Tyr490 is required for Shc association and activation of the Ras-MAP kinase cascade. Residues Tyr674/675 lie within the catalytic domain, and phosphorylation at this site reflects TrkA kinase activity (2-6). Point mutations, deletions and chromosomal rearrangements (chimeras) cause ligand-independent receptor dimerization and activation of TrkA. Many malignancies including breast, colon, prostate and thyroid carcinomas and acute myeloid leukemia have activated TrkA. Expression of TrkA in neuroblastomas is a good prognostic marker because it signals growth arrest and differentiation of cells originating from the neural crest (1).Phosphorylation at Tyr785 is required for activation of phospholipase Cγ and subsequent activation of the Ras-MAP kinase cascade. The phosphorylation site is conserved between TrkA and TrkB, as Tyr785 of TrkA corresponds to Tyr816 in TrkB of the human sequence (4,7).

Trk受体酪氨酸激酶家族包括TrkA,TrkB和TrkC。这些家庭成员的序列是高度保守的,它们是由不同的神经营养因子激活:NGF激活TrkA,BDNF或NT4激活TrkB,NT3激活TrkC。TrkA调节细胞增殖,神经系统的发育和成熟很重要(1)。在Tyr490磷酸化,需要与Shc结合及Ras-MAP激酶级联的激活。Tyr674/675残基在催化域内,此位点的磷酸化能影响TrkA的激酶活性(2-6)。点突变,缺失和染色体重排(嵌合体)会引起配体依赖受体的二聚化,激活TrkA。许多恶性肿瘤,包括乳腺癌、结肠癌、前列腺癌、甲状腺癌和急性髓细胞白血病有激活的TrkA。神经母细胞瘤的TrkA表达是一个预后良好的指标,因为它标志着肿瘤生长停滞和从神经嵴分化而来(1)。在Tyr785磷酸化,需要磷酸酶Cγ的激活,及后续Ras-MAP激酶级联的激活。TrkA和TrkB的磷酸化位点保守,TrkA的Tyr785对应人类TrkB序列的Tyr816(4,7)。

  1. Huang, E.J. and Reichardt, L.F. (2003) Annu Rev Biochem 72, 609-42.
  2. Segal, R.A. and Greenberg, M.E. (1996) Annu Rev Neurosci 19, 463-89.
  3. Stephens, R.M. et al. (1994) Neuron 12, 691-705.
  4. Marsh, H.N. et al. (2003) J Cell Biol 163, 999-1010.
  5. Obermeier, A. et al. (1993) EMBO J 12, 933-41.
  6. Obermeier, A. et al. (1994) EMBO J 13, 1585-90.
  7. Arevalo, J.C. et al. (2001) Oncogene 20, 1229-34.
  8. Reuther, G.W. et al. (2000) Mol Cell Biol 20, 8655-66.
  9. Greco, A. et al. (1997) Genes Chromosomes Cancer 19, 112-23.
  10. Pierotti, M.A. and Greco, A. (2006) Cancer Lett 232, 90-8.
  11. Lagadec, C. et al. (2009) Oncogene 28, 1960-70.
  12. Greco, A. et al. (2010) Mol Cell Endocrinol 321, 44-9.
  13. Ødegaard, E. et al. (2007) Hum Pathol 38, 140-6.
  14. Chou, T.T. et al. (2000) J Biol Chem 275, 565-70.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications