Cell Signaling Technology

Product Pathways - Apoptosis

TRAF5 (D3E2R) Rabbit mAb #41658

No. Size Price
41658S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
41658 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 64 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

TRAF5 (D3E2R) Rabbit mAb recognizes endogenous levels of total TRAF5 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His383 of human TRAF5 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using TRAF5 (D3E2R) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human TRAF5 (hTRAF5-Myc/DDK; +) using TRAF5 (D3E2R) Rabbit mAb (upper) or Myc-Tag (71D10) Rabbit mAb #2278 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from A172 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® TRAF5 siRNA I #60131 (+), or SignalSilence® TRAF5 siRNA II #38805 using TRAF5 (D3E2R) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The TRAF5 (D3E2R) Rabbit mAb confirms silencing of TRAF5 expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control.

Background

TRAFs (TNF receptor-associated factors) are a family of multifunctional adaptor proteins that bind to surface receptors and recruit additional proteins to form multiprotein signaling complexes capable of promoting cellular responses (1-3). Members of the TRAF family share a common carboxy-terminal "TRAF domain", which mediates interactions with associated proteins; many also contain amino-terminal Zinc/RING finger motifs. The first TRAFs identified, TRAF1 and TRAF2, were found by virtue of their interactions with the cytoplasmic domain of TNF-receptor 2 (TNFRII) (4). The six known TRAFs (TRAF1-6) act as adaptor proteins for a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress responses.

TRAF5 regulates signaling through binding to the cytoplasmic domains of TNFR famly members including CD40, CD27, CD30, OX40, and lymphotoxin-β receptor (5-10). Overexpression of TRAF5 induces NF-κB activation. Cytoplasmic aggregates of TRAF5, as well as TRAF2, were reported in Hodgkin-Reed-Sternberg cells, resulting in constitutive NF-κB activation (11).

Studies of TRAF5 deficient mice suggest that it plays an important role in limiting Th2 immune responses that triggers T-cell mediated inflammatory diseases and asthma (12). Further studies indicate that TRAF5 binds to the IL-6 receptor gp130 and negatively controls Th17 differentation (13). In B-cells, TRAF5 negatively regulates toll-like receptor (TLR) mediated cytokine and antibody production (14).

  1. Arch, R.H. et al. (1998) Genes Dev. 12, 2821-30.
  2. Chung, J. Y. et al. (2002) J. Cell Sci. 115, 679-88.
  3. Bradley, J.R. and Pober, J.S. (2001) Oncogene 20, 6482-91.
  4. Rothe, M. et al. (1994) Cell 78, 681-92.
  5. Nakano, H. et al. (1996) J Biol Chem 271, 14661-4.
  6. Ishida, T.K. et al. (1996) Proc Natl Acad Sci U S A 93, 9437-42.
  7. Aizawa, S. et al. (1997) J Biol Chem 272, 2042-5.
  8. Mizushima, S. et al. (1998) Gene 207, 135-40.
  9. Kawamata, S. et al. (1998) J Biol Chem 273, 5808-14.
  10. Nakano, H. et al. (1999) Proc Natl Acad Sci U S A 96, 9803-8.
  11. Horie, R. et al. (2002) Am J Pathol 160, 1647-54.
  12. So, T. et al. (2004) J Immunol 172, 4292-7.
  13. Nagashima, H. et al. (2014) Nat Immunol 15, 449-56.
  14. Buchta, C.M. and Bishop, G.A. (2014) J Immunol 192, 145-50.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

SignalSilence is a registered trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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