Cell Signaling Technology

Product Pathways - Neuroscience

EGR1 (44D5) Rabbit mAb #4154

AT225   Early growth response protein 1   EGR-1   Krox-24 protein   Nerve growth factor-induced protein A   NGFI-A   sc-189   Transcription factor ETR103   Transcription factor Zif268   Zinc finger protein 225   ZNF225  

No. Size Price
4154S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
4154 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 75 Rabbit IgG
IP 1:50
F 1:800
IF-IC 1:400
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry), ChIP=Chromatin IP,


Species predicted to react based on 100% sequence homology: Bovine,

Specificity / Sensitivity

EGR1 (44D5) Rabbit mAb detects endogenous levels of total EGR1 protein.

EGR1 (44D5) Rabbit mAb 兔单抗识别内源性的总EGR1蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of the sequence of human EGR1.


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of PC-12 cells, untreated (blue) or NGF treated (green), using EGR1 (44D5) Rabbit mAb compared to a nonspecific negative control (red).

流式细胞仪分析PC-12细胞,未处理(蓝色)或用NGF处理(绿色),使用的抗体是EGR1 (44D5) Rabbit mAb 兔单抗和非特异性阴性对照抗体(红色)。

Western Blotting

Western Blotting

Western analysis of cell extracts from serum starved PC12, untreated or NGF-treated (100 ng/ml for 2 hours), using EGR1 (44D5) Rabbit mAb.

Western blot分析PC12细胞提取物,不处理或100 ng/ml NGF转染2小时,使用的抗体是EGR1 (44D5)Rabbit mAb 兔单抗。



Confocal immunofluorescent analysis of dissociated PC12 cells either untreated (left) or NGF-treated for 2 hours (right), using EGR1 (44D5) Rabbit mAb (green) and MEK1/2 (L38C12) Mouse mAb (#4694) (red).

共聚焦免疫荧光分析分离的PC12细胞,未经处理(左图)或经过NGF处理2小时(右图),使用EGR1 (44D5) Rabbit mAb 兔单抗(绿色)和MEK1/2 (L38C12) Mouse mAb 鼠单抗#4694(红色)进行标记。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 THP-1 cells treated with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (30ng/ml) overnight and either 10 μl of EGR1 (44D5) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human ATAD2 promoter primers, SimpleChIP® Human EGR1 Promoter Primers #5549, human PCM1 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

使用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003试剂盒,将TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (30ng/ml)处理过夜的4 x 10^6 THP-1细胞的染色质,与10 μl EGR1 (44D5) Rabbit mAb 兔单抗或者2 μl of Normal Rabbit IgG #2729进行染色质免疫沉淀。使用人ATAD2启动子引物、SimpleChIP® Human EGR1 Promoter Primers #5549、人PCM1启动子引物和SimpleChIP® Human α Satellite Repeat Primers #4486进行real-time PCR用来定量富集的DNA。每个样本中被沉淀的DNA量用总投入量(等于1)的百分比表示。


EGR family members are transcriptional factors that contain three repetitive zinc finger DNA binding domains which bind to EGR response elements (ER) to regulate target gene expression (1). The expression of EGR family members is induced by growth factors, with EGR1 expression being induced by NGF (1,2). Increased EGR1 expression activates transcription of other signaling molecules, including CDK5 and tyrosine hydroxylase, and exerts long term effects on neural cell growth and differentiation (2,3).


  1. O'Donovan, K.J. et al. (1999) Trends Neurosci. 22, 167-173.
  2. Harada, T. et al. (2001) Nat. Cell Biol. 3, 453-459.
  3. Papanikolaou, N.A. and Sabban, E.L. (2000) J. Biol. Chem. 275, 26683-26689.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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