Cell Signaling Technology

Product Pathways - Neuroscience

EGR1 (15F7) Rabbit mAb #4153

AT225   Early growth response protein 1   EGR-1   Krox-24 protein   Nerve growth factor-induced protein A   NGFI-A   sc-110   sc-189   Transcription factor ETR103   Transcription factor Zif268   Zinc finger protein 225   ZNF225  

No. Size Price
4153S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
4153 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 75 Rabbit IgG
IP 1:50
IHC-P 1:50
IF-IC 1:800
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), ChIP=Chromatin IP,

Homology

Species predicted to react based on 100% sequence homology: Bovine,

Specificity / Sensitivity

EGR1 (15F7) Rabbit mAb detects endogenous levels of total EGR1 protein.

EGR1 (15F7)Rabbit mAb识别内源性的总EGR1蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of the sequence of human EGR1.

该单克隆抗体由合成的人类EGR1蛋白中氨基末端附近肽段免疫动物而制成。

Western Blotting

Western Blotting

Western analysis of extracts from serum starved PC12 cells, untreated or NGF-treated (100 ng/ml NGF for 2 hours), using EGR1 (15F7) Rabbit mAb.

Western blot分析PC12细胞提取液,不处理或100 ng/ml NGF转染2小时,使用的抗体是EGR1 (15F7)Rabbit mAb 兔单抗。

IF-IC

IF-IC

Confocal immunofluorescent analysis of dissociated PC12 cells either untreated (left) or NGF-treated for 2 hours (right) and labeled with EGR1 (44D5) Rabbit mAb (green) and MEK1/2 (L38C12) Mouse mAb #4694 (red).

共聚焦免疫荧光分析分离的PC12细胞,未经处理(左图)或经过NGF处理2小时(右图),使用的抗体是EGR1 (44D5) Rabbit mAb 兔单抗(绿色)和MEK1/2 (L38C12) Mouse mAb 鼠单抗#4694(红色)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse brain, using EGR1 (15F7) Rabbit mAb.

免疫组化分析石蜡包埋的小鼠大脑,使用的抗体是EGR1 (15F7) Rabbit mAb 兔单抗。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embeded human glioblastoma showing nuclear localization, using EGR1 (15F7) Rabbit mAb.

免疫组化分析石蜡包埋的人神经母细胞瘤,使用的抗体是EGR1 (15F7) Rabbit mAb 兔单抗,显示出核定位。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embeded human Non-Hodgkin's lymphoma, using EGR1 (15F7) Rabbit mAb.

免疫组化分析石蜡包埋的人非霍奇金淋巴瘤,使用的抗体是EGR1 (15F7) Rabbit mAb 兔单抗。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse brain using EGR1 (15F7) Rabbit mAb in the presence of control peptide (left) or EGR1 (15F7) Blocking Peptide #1015 (right).

免疫组织化学分析石蜡包埋的小鼠小脑,使用的抗体是EGR1 (15F7) Rabbit mAb 兔单抗,其中左图存在对照肽,右图存在EGR1(15F7) Blocking Peptide 阻断肽#1015。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 THP-1 cells treated with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (30ng/ml) overnight and either 10 μl of EGR1 (15F7) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human ATAD2 promoter primers, SimpleChIP® Human EGR1 Promoter Primers #5549, human PCM1 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

使用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003试剂盒,将TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (30ng/ml)处理过夜的4 x 10^6 THP-1细胞的染色质,与10 μl EGR1 (15F7) Rabbit mAb 兔单抗或者2 μl of Normal Rabbit IgG #2729进行染色质免疫沉淀。使用人ATAD2启动子引物、SimpleChIP® Human EGR1 Promoter Primers #5549、人PCM1启动子引物和SimpleChIP® Human α Satellite Repeat Primers #4486进行real-time PCR用来定量富集的DNA。每个样本被沉淀的DNA量用总投入量(等于1)的百分比表示。

Background

EGR family members are transcriptional factors that contain three repetitive zinc finger DNA binding domains which bind to EGR response elements (ER) to regulate target gene expression (1). The expression of EGR family members is induced by growth factors, with EGR1 expression being induced by NGF (1,2). Increased EGR1 expression activates transcription of other signaling molecules, including CDK5 and tyrosine hydroxylase, and exerts long term effects on neural cell growth and differentiation (2,3).

EGR的家庭成员是包含三个重复的锌指DNA结合域的转录因子,它可以EGR响应元素(ER),调节靶基因的表达(1)。EGR家族成员的表达可被生长因子诱导,EGR1的表达可被神经生长因子(NGF)诱导(1,2)。增加EGR1表达可以激活其他信号分子的转录,包括CDK5和酪氨酸羟化酶,对神经细胞生长和分化有长期影响(2,3)。

  1. O'Donovan, K.J. et al. (1999) Trends Neurosci. 22, 167-173.
  2. Harada, T. et al. (2001) Nat. Cell Biol. 3, 453-459.
  3. Papanikolaou, N.A. and Sabban, E.L. (2000) J. Biol. Chem. 275, 26683-26689.

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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