Cell Signaling Technology

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Phospho-Numb (Ser276) Antibody #4140

No. Size Price
4140S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4140 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 72, 74 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-Numb (Ser276) Antibody detects endogenous levels of Numb protein only when phosphorylated on Ser276.

Phospho-Numb (Ser276) 抗体只识别Ser276磷酸化的内源性Numb蛋白.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to Ser276 of the human Numb protein. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from Ramos and HeLa cells, untreated or treated with TPA (Phorbol-12-Myristate-13-Acetate, 200 nM for 30 min) or λ-phosphatase, using Phospho-Numb (Ser276) Antibody (upper) or Numb (C29G11) Rabbit mAb #2756 (lower).对经过TPA(Phorbol-12-Myristate-13-Acetate, 200 nM 30分钟)或λ-磷酸酶,处理或未处理的Ramos 和HeLa细胞提取物进行western blot分析,使用Phospho-Numb (Ser276) 抗体(上) or Numb (C29G11) Rabbit mAb #2756 (下).


Numb contains an amino-terminal phosphotyrosine-binding (PTB) domain and carboxy-terminal endocytic binding motifs for α-adaptin and EH (Eps15 homology) domain-containing proteins, indicating a role in endocytosis (1,2). There are four mammalian Numb splicing isoforms that are differentially expressed and may have distinct functions (3-5). Numb acts as a negative regulator of Notch signaling by promoting ubiquitination and degradation of Notch (6). The protein is asymmetrically segregated into one daughter cell during cell division, producing two daughter cells with different responses to Notch signaling and different cell fates (7,8). The localization of Numb can also be regulated by G-protein coupled receptor (GPCR) and PKC signaling (9).

Numb包含一个氨基末端磷酸化酪氨酸结合(PTB)结构域和C末端对α-衔接蛋白和EH(Eps15 同源物)结构域包含蛋白的内吞结合基序,显示在细胞吞噬中的作用(1,2)有四个哺乳动物Numb剪接异构体,差异表达,并有各自独特功能(3-5)。Numb通过促进Notch泛素降解对Notch信号通路起负向调控作用(6),蛋白在细胞分裂时不对称的入子细胞,产生的两个子细胞对Notch信号和细胞命运都有不同反应(7,8)。Numb可能被G蛋白偶联受体和PKC信号调控(9)。

Numb can be phosphorylated at several sites including Ser7, Ser276 and Ser295. Phosphorylation at these sites regulates asymmetric membrane localization of Numb and integrin endocytosis (10-12).


  1. Berdnik, D. et al. (2002) Dev. Cell 3, 221-231.
  2. Santolini, E. et al. (2000) J. Cell Biol. 151, 1345-1352.
  3. Dho, S.E. et al. (1999) J. Biol. Chem. 274, 33097-33104.
  4. Verdi, J.M. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 10472-10476.
  5. Toriya, M. et al. (2006) Dev. Neurosci. 28, 142-155.
  6. McGill, M.A. and McGlade, C.J. (2003) J. Biol. Chem. 278, 23196-23203.
  7. Verdi, J.M. et al. (1996) Curr. Biol. 6, 1134-1145.
  8. Reugels, A.M. et al. (2006) Dev. Dyn. 235, 934-948.
  9. Dho, S.E. et al. (2006) Mol. Biol. Cell 17, 4142-4155.
  10. Nishimura, T. and Kaibuchi, K. (2007) Dev Cell 13, 15-28.
  11. Smith, C.A. et al. (2007) EMBO J. 26, 468-480.
  12. Wirtz-Peitz, F. et al. (2008) Cell 135, 161-173.

Application References

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