Cell Signaling Technology

Product Pathways - Metabolism

Phospho-HSL (Ser563) Antibody #4139

hormone-sensitive-lipase  

No. Size Price
4139S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4139T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
4139 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Mouse, Endogenous 81, 83 Rabbit
IF-IC 1:800

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry),

Homology

Species predicted to react based on 100% sequence homology: Human, Rat,

Specificity / Sensitivity

Phospho-HSL (Ser563) Antibody detects endogenous levels of HSL only when phosphorylated at Ser563 by PKA. This antibody does not cross-react with Ser565 phosphorylated HSL.

Phospho-HSL (Ser563) Antibody用于检测内源性的在Ser563被PKA磷酸化的HSL蛋白水平,该抗体不与Ser565磷酸化的HSL蛋白交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser552 of human HSL (equivalent to Ser563 of rat HSL). Antibodies are purified by protein A and peptide affinity chromatography.

多克隆抗体是通过合成对应于人HSL蛋白Ser552(大鼠HSL的Ser563)序列的肽段免疫动物获得,抗体经Protein A和肽亲和层析纯化制得。

Western Blotting

Western Blotting

Western blot analysis of extracts from differentiated 3T3-L1 cells treated with isoproterenol or lambda protein phosphatase, using Phospho-HSL (Ser563) Antibody.

western blot检测分化的3T3-L1细胞提取物,细胞用异丙肾上腺素或lambda蛋白磷酸酶处理,所用抗体为 Phospho-HSL (Ser563) Antibody。

IF-IC

IF-IC

Confocal immunofluorescent analysis of 3T3-L1 adipocytes, isoproterenol-treated (left) or phosphatase-treated (right), labeled with Phospho-HSL (Ser563) Antibody (red). Lipid droplets have been labeled with BODIPY 493/503 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

激光共聚焦荧光免疫检测3T3-L1细胞,细胞未处理(左)或用磷酸酶处理(右),用Phospho-HSL (Ser563) Antibody(红)标记,显示在分化细胞中位于胞浆中。脂质滴用BODIPY® 493/503 (绿)标记。蓝色伪彩为DNA荧光染料(对应产品号为DRAQ5® #4084)。

Background

HSL (hormone-sensitive lipase) catalyzes the hydrolysis of triacylglycerol, the rate-limiting step in lipolysis. Lipolytic stimuli activate adenylyl cyclase and thus increase intracellular cAMP levels, which in turn activate protein kinase A (PKA). PKA phosphorylates HSL at Ser563, Ser659 and Ser660, which stimulates HSL activity (1,2). In contrast, AMPK phosphorylates HSL at Ser565, which reduces HSL phosphorylation at Ser563 by PKA and inhibits HSL activity (2,3). Recent work indicates that phosphorylation at Ser600 by p44/42 MAPKs also enhances the enzymatic activity of HSL (4).

激素敏感型脂肪酶(HSL)催化甘油三酯的水解反应,这是脂肪分解过程的限速步骤。脂分解性的刺激因素会激活腺苷酸环化酶,因而增加了胞内cAMP的水平,进一步会增加蛋白激酶A的活性。 PKA会磷酸化HSL的 Ser563, Ser659 和Ser660,磷酸化会激活HSL(1,2)。相反,AMPK可以磷酸化HSL的Ser565,这会阻止HSL被PKA对其Ser563磷酸化,这会抑制HSL的活性(2,3)。最近的研 究表明,p44/42 MAPKs 对HSL Ser600的磷酸化也会增加它的活性(4)。

  1. Degerman, E. et al. (1990) Proc. Natl. Acad. Sci. USA 87, 533-37.
  2. Anthonsen, M.W. et al. (1998) J. Biol. Chem. 273, 215-21.
  3. Garton, A.J. and Yeaman, S.J. (1990) Eur. J. Biochem. 191, 245-50.
  4. Greenberg, A.S. et al. (2001) J. Biol. Chem. 276, 45456-61.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

BODIPY is a registered trademark of Life Technologies Corporation.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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