Cell Signaling Technology

Product Pathways - Metabolism

MTHFD2 (D8W9U) Rabbit mAb #41377

sc-390708  

No. Size Price
41377S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
41377 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 35 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

MTHFD2 (D8W9U) Rabbit mAb recognizes endogenous levels of total MTHFD2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu340 of human MTHFD2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and 293 cells using MTHFD2 (D8W9U) Rabbit mAb.

Background

MTHFD2 is a bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase involved in mitochondrial folate metabolism (1). MTHFD2 expression is developmentally regulated, as it is expressed in embryos but not in most adult tissues. Recent research studies have shown that MTHFD2 is consistently overexpressed in many cancer types and correlated with poor survival in breast cancer (2-5). Overexpression of MTHFD2 promotes cell proliferation while its depletion induces cell death in human cancer cells (6).

  1. Christensen, K.E. and Mackenzie, R.E. (2008) Vitam Horm 79, 393-410.
  2. Lehtinen, L. et al. (2013) Oncotarget 4, 48-63.
  3. Nilsson, R. et al. (2014) Nat Commun 5, 3128.
  4. Liu, F. et al. (2014) Tumour Biol 35, 8685-90.
  5. Tedeschi, P.M. et al. (2015) Mol Cancer Res 13, 1361-6.
  6. Gustafsson Sheppard, N. et al. (2015) Sci Rep 5, 15029.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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