Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Phospho-Cyclin E1 (Thr62) Antibody #4136

No. Size Price
4136S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4136 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 48 Rabbit
IP 1:100
IHC-P 1:100
F 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry,

Specificity / Sensitivity

Phospho-Cyclin E (Thr62) Antibody detects endogenous levels of cyclin E only when phosphorylated at threonine 62 (cyclin E1 isoform 2) or threonine 77 (cyclin E1 isoform 1). Phospho-Cyclin E (Thr62) Antibody能够检测内源性的苏氨酸62位点(cyclin E1 isoform 2)或苏氨酸77位点(cyclin E1 isoform 1)磷酸化的cyclin E蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr62 of human cyclin E. Antibodies are purified by protein protein A and peptide affinity chromatography. 该多克隆抗体是由合成的人源的针对cyclin E蛋白苏氨酸(62位)磷酸化肽段免疫动物,利用A蛋白和多肽亲和层析方法纯化生产的。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of untreated Jurkat cells, using Phospho-Cyclin E1 (Thr62) Antibody versus propidium iodide (DNA content). The boxed population indicates phospho-Cyclin E (Thr62)-positive cells.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing nuclear localization, using Phospho-Cyclin E (Thr62) Antibody. 免疫组织化学方法检测石蜡包埋人类结肠癌组织,显示核定位,检测抗体为Phospho-Cyclin E (Thr62) Antibody。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-Cyclin E (Thr62) Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-Cyclin E (Thr62) Antibody in the presence of control peptide (left) or antigen-specific peptide (right). 免疫组织化学方法检测石蜡包埋人类乳腺癌组织,左图为对照肽段,右图为抗原特异性肽段,检测抗体为Phospho-Cyclin E (Thr62) Antibody。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells, treated with 10 mM LiCl for the indicated times, using Phospho-Cyclin E1 (Thr62) Antibody (upper) or a cyclin E antibody (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells, untreated or hydroxyurea-treated, using Phospho-Cyclin E1 (Thr62) Antibody.

Background

Cyclin E1 and cyclin E2 can associate with and activate CDK2 (1). Upon DNA damage, upregulation/activation of the CDK inhibitors p21 Waf1/Cip1 and p27 Kip1 prevent cyclin E/CDK2 activation, resulting in G1/S arrest. When conditions are favorable for cell cycle progression, cyclin D/CDK4/6 phosphorylates Rb and is thought to reduce the activity of p21 Waf1/Cip1 and p27 Kip1, allowing subsequent activation of cyclin E/CDK2 (1,2). Cyclin E/CDK2 further phosphorylates Rb to allow progression into S-phase, where cyclin E/CDK2 is thought to phosphorylate and activate multiple proteins involved in DNA synthesis (2,3). Turnover of cyclin E is largely controlled by phosphorylation that results in SCFFbw7-mediated ubiquitination and proteasome-dependent degradation (4,5). Cyclin E1 is phosphorylated at multiple sites in vivo including Thr62, Ser88, Ser72, Thr380 and Ser384, and is controlled by at least two kinases, CDK2 and GSK-3 (6,7). Cyclin E1和Cyclin E2协同并激活CDK2(1)。DNA损伤时,上调/激活CDK抑制基因p21 Waf1/Cip1 and p27 Kip1能够阻滞cyclin E/CDK2的激活,从而导致G1/S期停滞。当条件适宜于细胞周期连续进行时,cyclin D/CDK4/6使Rb磷酸化从而能够降低p21 Waf1/Cip1 和 p27 Kip1的活性,使得cyclin E/CDK2随后激活(1,2)。Cyclin E/CDK2进一步磷酸化Rb使细胞周期进入S期,在此期内cyclin E/CDK2被认为可以磷酸化并激活涉及DNA合成过程的多种蛋白(2,3)。周期蛋白E的功能转化主要受磷酸化控制,这种磷酸化能够导致SCFFbw7介导的泛素化和蛋白酶体依赖的降解(4,5)。Cyclin E1在体内可以在包括苏氨酸62位,丝氨酸88位,丝氨酸72位,苏氨酸380位和丝氨酸384位的多位点磷酸化,这个磷酸化过程至少可以被两个激酶-CDK2和GSK-3调控。

  1. Lauper, N. et al. (1998) Oncogene 17, 2637-43.
  2. Lundberg, A.S. and Weinberg, R.A. (1998) Mol Cell Biol 18, 753-61.
  3. Ewen, M.E. (2000) Genes Dev 14, 2265-70.
  4. Won, K.A. and Reed, S.I. (1996) EMBO J 15, 4182-93.
  5. Koepp, D.M. et al. (2001) Science 294, 173-7.
  6. Welcker, M. et al. (2003) Mol Cell 12, 381-92.
  7. Ye, X. et al. (2004) J Biol Chem 279, 50110-9.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications