Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Phospho-Stat4 (Tyr693) (D2E4) Rabbit mAb #4134


No. Size Price
4134S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4134 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 81 Rabbit IgG
IP 1:100
F 1:800
IF-IC 1:800
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry), ChIP=Chromatin IP,


Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey, Pig,

Specificity / Sensitivity

Phospho-Stat4 (Tyr693) (D2E4) Rabbit mAb recognizes endogenous levels of Stat4 protein only when phosphorylated at Tyr693.

Phospho-Stat4 (Tyr693) (D2E4) Rabbit mAb 兔单抗只能检测内源的在tyr693位点磷酸的Stat4蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr693 of human Stat4 protein.

此单克隆抗体是通过合成人源对应的Stat4 Tyr693位点周围的多肽段来免疫动物而获得。



Confocal immunofluorescent analysis of NK-92 cells, starved of IL-2 (5 hr) and then either untreated (upper) or IL-12-treated (50 ng/mL, 15 min; lower), using Phospho-Stat4 (Tyr693) (D2E4) Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red).共聚焦免疫荧光分析经IL-2饥饿 5 小时 NK-92细胞,未经处理 (上图) 和进过IL-12(50 ng/mL, 15 min) 处理(下图),Phospho-Stat4 (Tyr693) (D2E4) Rabbit mAb(绿色)和 β-Actin (8H10D10) Mouse mAb #3700(红色)。

Western Blotting

Western Blotting

Western blot analysis of extracts from NK-92 cells, untreated (-) or treated (+) with Human Interleukin-2 (hIL-2) #8907 (10 ng/ml, 15 min) or human interleukin-12 (hIL-12, 50 ng/ml, 15 min), using Phospho-Stat4 (Tyr693) (D2E4) Rabbit mAb (upper) and Stat4 (C46B10) Rabbit mAb #2653 (lower).Western免疫印迹。用Phospho-Stat4 (Tyr693) (D2E4) Rabbit mAb (上图) 和Stat4 (C46B10) Rabbit mAb #2653 (下图) 研究未经处理的和经(-) 或 经过 hIL-2 #8907 (10 ng/ml, 15 min) 或者(hIL-12, 50 ng/ml, 15 min)处理(+) 的NK-92 细胞的细胞提取液。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NK-92 cells starved of IL-2 overnight then treated with IL-12 (10 ng/ml, 4 hr) and either 20 μl of Phospho-Stat4 (Tyr693) (D2E4) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human IRF-1 promoter primers, SimpleChIP® Human PRF1 Promoter Primers #9014, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.染色质免疫共沉淀。NK-92细胞培养至4 x 106,IL-2饥饿过夜并经IL-12 (10 ng/ml)处理4小时,然后用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003进行免疫沉淀实验,所用的抗体为20 μl Phospho-Stat4 (Tyr693) (D2E4) Rabbit mAb或2 μl of Normal Rabbit IgG #2729,本实验中用IRF-1 promoter primers, SimpleChIP® Human PRF1 Promoter Primers #9014和 SimpleChIP® Human α Satellite Repeat Primers #4486引物对富集的DNA做real-time PCR。每个样本中沉淀的DNA量定义为相对信号与输入的总染色质相比的数值。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of NK-92 cells, untreated (blue) or treated with IL-12 (green), using Phospho-Stat4 (Tyr693) (D2E4) Rabbit mAb.流式细胞仪分析NK-92 细胞,未经处理(蓝色) 或经过IL-12处理(绿色) ,所用抗体为Phospho-Stat4 (Tyr693) (D2E4) Rabbit mAb。


The Jak-Stat signaling pathway is utilized by a large number of cytokines, growth factors, and hormones (1). Receptor-mediated tyrosine phosphorylation of Jak family members triggers phosphorylation of Stat proteins, resulting in their nuclear translocation, binding to specific DNA elements, and subsequent activation of transcription. The remarkable range and specificity of responses regulated by the Stats is determined, in part, by the tissue-specific expression of different cytokine receptors, Jaks, and Stats, as well as by the combinatorial coupling of various Stat members to different receptors (2). Stat4 is predominantly expressed in the spleen, thymus, and testis and has been most extensively investigated as the mediator of IL-12 responses (3-8). Activation of Stat4 is associated with phosphorylation at Tyr693 (9).

Jak-Stat信号通路被很多的细胞因子,生长因子和激素所利用(1)。受体诱导的 Jak家族蛋白的酪氨酸磷酸化引起Stat蛋白的磷酸化导致他们入核,与DNA原件结合并活化转录。Stats所调控反应显著的专一性和范围一部分受控于组织特异性表达不同的细胞因子受体,Jaks 和 Stats同时也与不同的Stat成员偶联到不同的受体上的组合有关(2)。Stat4主要是在脾、胸腺和睾丸组织中表达,并且作为IL-12的调节蛋白而得到研究(3-8)。Stat4的激活与Tyr693位点的磷酸化有关(9)。

Stat4 is activated in response to IL-2 in natural killer (NK) cells, but not in T cells (10).


  1. Darnell, J.E. et al. (1994) Science 264, 1415-1421.
  2. Leonard, W.J. and O'Shea, J.J. (1998) Annu. Rev. Immunol. 16, 293-322.
  3. Zhong, Z. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 4806-4810.
  4. Yamamoto, K. et al. (1994) Mol. Cell Biol. 14, 4342-4349.
  5. Jacobson, N.G. et al. (1995) J. Exp. Med. 181, 1755-1762.
  6. Bacon, C.M. et al. (1995) Proc. Natl. Acad. Sci. USA 92, 7307-7311.
  7. Thierfelder, W.E. et al. (1996) Nature 382, 171-174.
  8. Kaplan, M.H. et al. (1996) Nature 382, 174-177.
  9. Visconti, R. et al. (2000) Blood 96, 1844-52.
  10. Wang, K.S. et al. (1999) J Immunol 162, 299-304.

Application References

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