Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Phospho-Cyclin B1 (Ser133) (9E3) Rabbit mAb #4133

No. Size Price
4133S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4133 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 55 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-Cyclin B1 (Ser133) (9E3) Rabbit mAb detects endogenous levels of cyclin B1 protein only when phosphorylated at Ser133. Phospho-Cyclin B1 (Ser133) (9E3) Rabbit mAb 能够检测内源性的丝氨酸(133位点)磷酸化的cyclin B1蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser133 of human cyclin B1. 该单克隆抗体是由合成的人源的针对cyclin B1蛋白丝氨酸(133位)磷酸化肽段免疫动物而生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, treated with hydroxyurea or nocodazole for 24 hours, using Phospho-Cyclin B1 (Ser133) (9E3) Rabbit mAb (upper) or Cyclin B1 (U152) Mouse mAb #4135 (lower). western blot方法检测用羟基脲和nocodazole处理24小时的HeLa细胞提取物。使用的抗体为 Phospho-Cyclin B1 (Ser133) (9E3) Rabbit mAb(上图) 和 Cyclin B1 (U152) Mouse mAb #4135(下图).

Background

Entry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. Activation of cdc2 is controlled at several steps including cyclin B1 binding, phosphorylation of cdc2 at Thr161 and dephosphorylation of cdc2 at Thr14/Tyr15 (1-5). The protein levels of CDK inhibitors and the CDK-associated cyclins are regulated by phosphorylation, ubiquitination and degradation, allowing for a stoichiometric regulation of cell cycle events (6). Four cyclin B1 phosphorylation sites (Ser126, 128, 133 and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint (8-10). Phosphorylation of cyclin B1 is required for cdc25C-dependent dephosphorylation of Tyr15 within cdc2 and subsequent cdc2/cyclin B1 activation (11). While cdc2/cyclin B1 itself can phosphorylate Ser126 and Ser128 (8), polo-like kinase 1 phosphorylates cyclin B1 preferentially at Ser133 and possibly at Ser147 as well (11-13). cdc2蛋白激酶的激活参与调节所有的真核细胞进入有丝分裂。cdc2的激活受多个步骤的调控,包括周期蛋白B的结合、cdc2苏氨酸161位的磷酸化和苏氨酸14位和15位的去磷酸化(1-5)。CDK抑制剂和CDK相关的细胞周期蛋白的水平可通过磷酸化、泛素化和降解来调节,允许了细胞周期事件的化学计量调节(6)。四个周期蛋白B1磷酸化位点(丝氨酸126位,128位,133位和147位)位于胞质滞留信号(CRS)结构域,并可以调节G2/S检验点周期蛋白B1的核迁移(8-10)。周期蛋白B1的磷酸化参与了cdc25C依赖的cdc2 酪氨酸15位的去磷酸化和随后的cdc2/周期蛋白B1 激活(11)。尽管cdc2/周期蛋白B1本身能够磷酸化丝氨酸126位和128位(8),polo样蛋白激酶优先磷酸化丝氨酸133位,也可能磷酸化丝氨酸147位(11-13)。

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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