Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

Histone H1.4 (D4J5Q) Rabbit mAb #41328

sc-393358  

No. Size Price
41328S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
41328 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 30 Rabbit IgG
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, ChIP=Chromatin IP,

Homology

Species predicted to react based on 100% sequence homology: Hamster, Bovine, Dog,

Specificity / Sensitivity

Histone H1.4 (D4J5Q) Rabbit mAb recognizes endogenous levels of total histone H1.4 protein. This antibody also cross reacts with histone H1.5 (UniProt P16401) and weakly with histones H1.1 (UniProt Q02539), H1.2 (UniProt P16403), and H1.3 (UniProt P16402).

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala176 of human histone H1.4 protein.

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HeLa cells and either 10 μl of Histone H1.4 (D4J5Q) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human GAPDH Exon 1 Primers #5516, SimpleChIP® Human γ-Actin Promoter Primers #5037, SimpleChIP® Human RPL30 Exon 3 Primers #7014, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T, HCT 116, and COS-7 cells using Histone H1.4 (D4J5Q) Rabbit mAb.

Background

Histones are evolutionarily conserved proteins that play a vital role in the compaction, storage, and regulation of DNA within the eukaryotic nucleus. The basic subunit of chromatin, the nucleosome core particle, is composed of DNA wound around two copies each of the core histone proteins H2A, H2B, H3, and H4 (1-3). Formation of higher order chromatin structure is facilitated through the binding of linker histone H1 to the nucleosome particle (chromatosome) (4-6). In humans and mice, there are 11 distinct histone H1 variants, which include the somatic variants (H1.1, H1.2, H1.3, H1.4, and H1.5) that are expressed ubiquitously, and cell type specific variants such as H1t found in the testis and H1.0 expressed in terminally differentiated cells (6-10). Binding of histone H1 to chromatin limits accessibility of DNA to other proteins by stabilizing nucleosome positioning, competing for binding sites, and limiting the activity of chromatin remodeling proteins such as the SWI/SNF complex (6,11-14). Histone H1 binding is highly dynamic and is thought to be regulated by post-translational modifications (6). For example, cell cycle regulated phosphorylation of histone H1 leads to chromatin condensation and decondensation depending on the site of phosphorylation and histone H1.4 Lys34 acetylation by GCN5 has been linked to increased mobility of H1.4 and transcriptional activation (6, 15-18).

  1. Kornberg, R.D. (1974) Science 184, 868-71.
  2. Kornberg, R.D. and Thomas, J.O. (1974) Science 184, 865-8.
  3. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
  4. Thoma, F. et al. (1979) J Cell Biol 83, 403-27.
  5. Carruthers, L.M. et al. (1998) Biochemistry 37, 14776-87.
  6. Harshman, S.W. et al. (2013) Nucleic Acids Res 41, 9593-609.
  7. Eick, S. et al. (1989) Eur J Cell Biol 49, 110-5.
  8. Carozzi, N. et al. (1984) Science 224, 1115-7.
  9. Drabent, B. et al. (1991) Gene 103, 263-8.
  10. Pehrson, J.R. and Cole, R.D. (1982) Biochemistry 21, 456-60.
  11. Bustin, M. et al. (2005) Mol Cell 17, 617-20.
  12. Hill, D.A. and Imbalzano, A.N. (2000) Biochemistry 39, 11649-56.
  13. Ramachandran, A. et al. (2003) J Biol Chem 278, 48590-601.
  14. Horn, P.J. et al. (2002) Nat Struct Biol 9, 263-7.
  15. Hohmann, P. et al. (1976) J Biol Chem 251, 3685-92.
  16. D'Anna, J.A. et al. (1978) Nucleic Acids Res 5, 3195-207.
  17. Matsumoto, Y. et al. (1980) Nature 284, 181-3.
  18. Kamieniarz, K. et al. (2012) Genes Dev 26, 797-802.

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For Research Use Only. Not For Use In Diagnostic Procedures.

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