Cell Signaling Technology

Product Pathways - DNA Damage

Phospho-TLK1 (Ser743) Antibody #4121

tousled  

No. Size Price
4121S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4121 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 86 Rabbit
IP 1:100
IHC-P 1:300

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

Phospho-TLK1 (Ser695) Antibody detects endogenous levels of TLK1 only when phosphorylated at serine 695.Phospho-TLK1 (Ser695) Antibody能够检测内源性丝氨酸(695位)磷酸化的TLK1蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser695 of human TLK1. Antibodies are purified by protein A and peptide affinity chromatography.该多克隆抗体是由合成的人源的针对TLK1蛋白丝氨酸(695位)的磷酸化肽段免疫动物,采用蛋白A和多肽亲和层析技术纯化生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or treated overnight with hydroxyurea or aphidicolin, using Phospho-TLK1 (Ser743) Antibody (upper), or TLK1 Antibody #4125 (lower).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, untreated (left) or lambda phosphatase-treated (right), using Phospho-TLK1 (Ser695) Antibody.免疫组织化学方法检测石蜡包埋人肺癌组织,分为非处理组(左图)或lambda磷酸酶处理组(右图),使用的抗体为Phospho-TLK1 (Ser695) Antibody。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma, using Phospho-TLK1 (Ser695) Antibody in the presence of control peptide (left) or antigen-specific peptide (right).免疫组织化学方法检测石蜡包埋人肾细胞癌组织,使用的抗体为Phospho-TLK1 (Ser695) Antibody,左图为共孵育对照多肽,右图为共孵育抗原特异性多肽。

Background

Tousled-like kinases (TLK1 and TLK2) are nuclear serine/threonine kinases named for their homology to the Tousled gene from Arabidopsis thaliana, essential for flower development (1). The kinase activities of the TLKs are cell cycle regulated, with maximal activity during S phase (1). TLK appears to play a role in chromatin assembly and DNA damage checkpoint regulation (1,2). In C. elegans, TLK1 is essential for appropriate transcription during embryonic development (3). Substrates for TLK include the human chromatin assembly factor Asf, which functions in DNA replication- and repair-coupled chromatin assembly (2). DNA damage during S phase, when TLK is maximally active, leads to inhibition of TLK activity (1). This inhibition requires ataxia mutated kinase (ATM) and Chk1 (4,5). ATM and the related kinase ATR are activited by DNA damage during S phase, phosphorylate Chk1/Chk2, and block the transition into mitosis (6). Chk1 phosphorylates TLK1 on Ser695 in vitro and in vivo, leading to inhibition of TLK1 activity (4). This process likely provides a mechanism to slow the chromatin assembly processes controlled by TLK in the event of DNA damage. Tousled样激酶(TLK1 and TLK2)属于核丝氨酸/苏氨酸激酶,因其与来自拟南芥的Tousled基因的同源性而得名,对于花的发育必不可少(1)。TLKs的激酶活性是细胞周期调控的,在S期活性最高(1)。TLK似乎在染色质装配和DNA损伤检验点调控中起重要作用(1,2)。TLK1对于线虫胚胎发育过程中的适当转录是必需的(3)。TLK的底物包括人类染色质装配因子Asf,它在DNA复制和修复偶联染色质装配过程中起作用(2)。S期DNA损伤时,TLK被最大程度的活化,导致TLK活性抑制(1)。这种抑制需要ATM和Chk1(4,5)。S期DNA损伤激活ATM和相关激酶ATR,使Chk1/Chk2磷酸化,阻遏有丝分裂期的转换(6)。Chk1体内/外都可以磷酸化TLK1的695位丝氨酸,引起TLK1的活性抑制(4)。该过程很可能提供了一种在DNA损伤事件中减慢TLK控制的染色质装配过程。

  1. Sillje, H. H. et al. (1999) EMBO J. 18, 5691-5702.
  2. Sillje, H.H. and Nigg, E.A. (2001) Curr. Biol. 11, 1068-1073.
  3. Han, Z. et al. (2003) Curr. Biol. 13, 1921-1929.
  4. Groth, A. et al. (2003) EMBO J. 22, 1676-1687.
  5. Krause, D. R. et al. (2003) Oncogene 22, 5927-5937.
  6. Kastan, M.B. and Lim, D.S. (2000) Nat. Rev. Mol. Cell Biol. 1, 179-186.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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