Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb #4113

pstat3   sc-8059  

No. Size Price
4113S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4113 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:2000 Human,Mouse,Rat,Monkey, Endogenous 79, 86 Mouse IgG1
IP 1:100
IHC-P 1:100
F 1:100
IF-IC 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb detects endogenous levels of Stat3 only when phosphorylated at Tyr705. This antibody does not cross-react with phospho-EGFR or the corresponding phospho-tyrosines of other Stat proteins.

Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb 只能检测内源的在tyr705位点磷酸的 Stat3 蛋白。此抗体不与磷酸化的EGFR和对应的磷酸化酪氨酸残基的其它Stat 蛋白反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr705 of mouse Stat3.

此单克隆抗体是通过合成鼠源对应的Stat3 Tyr705位点周围的磷肽段来免疫动物而获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or treated with IFN-α (100 ng/ml) for 5 minutes, using Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb (left) or Stat3 Antibody #9132 (right).Western免疫印迹。用 Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb (左图) 或Stat3 Antibody #9132 (右图).研究未经处理的和经IFN-alpha(100 ng/ml,5 min)处理的 HeLa 细胞的细胞提取液。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, untreated (blue) or IFN-α treated (green), using Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb.流式细胞仪研究未经处理的Jurkat细胞(蓝色)和经IFN-α处理的Jurkat细胞(绿色)。所用抗体为Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical anlysis of paraffin-embedded human breast carcinoma using Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb.免疫组织化学染色分析石蜡包埋人乳腺癌组织。所用抗体为Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, untreated (left) or IFN-alpha treated (right), using Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb (green) and β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116 (red).共聚焦免疫荧光分析未经处理的HeLa(左图)或者经IFN-α 处理的 HeLa (右图),所用抗体为Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb (绿色) 和 β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116 (红色)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis using Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb on SignalSlide® Phospho-Stat1/3/5 IHC Controls #8105 (paraffin-embedded HeLa cell pellets, untreated (left), treated with Human Interferon-α1 (hIFN-α1) #8927 (middle), or treated with Human Epidermal Growth Factor (hEGF) #8916 (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis using Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb on SignalSlide® HeLa -/+ IFNa IHC Controls #55861 (paraffin-embedded HeLa cell pellets, untreated (left) or treated with Human Interferon-α1 (hIFN-α1) #8927 (right)).

Background

The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).

Stat3 转录因子是细胞因子和生长因子受体信号通路上重要的信号分子(1),并且被鼠科胚胎的发育所需要(2)。Stat3 在很多的人类肿瘤中是组成性激活的(3,4)并有潜在的支配原发性癌的可能(5) 而且也具有抗细胞凋亡的活性(3)。Stat3通过Tyr705位点的磷酸化而激活,从而导致它的二聚化,入核和DNA结合(6,7)。转录活性似乎受到 MAPK 或 mTOR 信号通路介导的Stat3 Ser727位点的磷酸化(8,9)。Stat3不同剪切形式的表达反应了其生物学功能,Stat3α (86 kDa) 和 Stat3β (79 kDa) 形式的表达比例依赖于细胞类型,配体方向或者细胞发育的成熟阶段(10)。值得注意的是Stat3β蛋白在C-末端转录活性区域缺少丝氨酸磷酸化位点(8)。

  1. Heim, M.H. (2001) J Recept Signal Transduct Res 19, 75-120.
  2. Takeda, K. et al. (1997) Proc Natl Acad Sci U S A 94, 3801-4.
  3. Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15.
  4. Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85.
  5. Bromberg, J.F. et al. (1999) Cell 98, 295-303.
  6. Darnell, J.E. et al. (1994) Science 264, 1415-21.
  7. Ihle, J.N. (1995) Nature 377, 591-4.
  8. Wen, Z. et al. (1995) Cell 82, 241-50.
  9. Yokogami, K. et al. (2000) Curr Biol 10, 47-50.
  10. Biethahn, S. et al. (1999) Exp Hematol 27, 885-94.

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