Cell Signaling Technology

Product Pathways - Metabolism

MICAL1 (D8K7R) Rabbit mAb #41112

No. Size Price
41112S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
41112 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 130 Rabbit IgG
IP 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

MICAL1 (D8K7R) Rabbit mAb recognizes endogenous levels of total MICAL1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val1038 of human MICAL1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MICAL1 (D8K7R) Rabbit mAb.



Immunoprecipitation of MICAL1 from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is MICAL1 (D8K7R) Rabbit mAb. Western blot analysis was performed using MICAL1 (D8K7R) Rabbit mAb.


Molecule interacting with CasL protein 1 (MICAL1) is a Protein-methionine sulfoxide oxidase. MICAL1 can bind directly to F-actin and oxidize specific methionine residues to promote actin filament disassembly (1-3). MICAL1 is an important component of semaphorin signaling cascades that has effects on cell movement, angiogenesis, immunology, diabetes, and cancer (4-7). MICAL1 binds to NDR1/2 and antagonizes MST1-induced NDR activation and apoptosis (8).

  1. Hung, R.J. et al. (2013) Nat Cell Biol 15, 1445-54.
  2. Lee, B.C. et al. (2013) Mol Cell 51, 397-404.
  3. Giridharan, S.S. and Caplan, S. (2014) Antioxid Redox Signal 20, 2059-73.
  4. Terman, J.R. et al. (2002) Cell 109, 887-900.
  5. Aggarwal, P.K. et al. (2015) Diabetes 64, 1743-59.
  6. Loria, R. et al. (2015) Oncotarget 6, 2779-93.
  7. Zhou, Y. et al. (2011) Mol Cell Biol 31, 3603-15.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :